Protease, Serine, 7 (Enterokinase) (PRSS7) ELISA Kit for Human

Name: Human PRSS7 ELISA Kit; Protease, Serine, 7 (Enterokinase); MyBioSource
Brand: MyBioSource
SKU: MBS920466
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS920466

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRSS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRSS7 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

protease, serine, 7 (enterokinase) (PRSS7), ELISA Kit

Full Product Name

Human Enteropeptidase, PRSS7 ELISA Kit

Product Gene Name

PRSS7 elisa kit

Product Synonym Names

Human Enteropeptidase (PRSS7) ELISA kit; ENTK; MGC133046; enterokinase; enteropeptidase; protease; serine; 7 (enterokinase)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.312 ng/ml-20 ng/ml

Sensitivity

< 0.078 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human PRSS7. No significant cross-reactivity or interference between human PRSS7 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

112,935 Da

OMIM

606635

3D Structure

ModBase 3D Structure for P98073

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PRSS7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRSS7 purchase

MBS920466 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protease, serine, 7 (enterokinase) (PRSS7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRSS7. The ELISA analytical biochemical technique of the MBS920466 kit is based on PRSS7 antibody-PRSS7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRSS7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRSS7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PRSS7. It may not necessarily be applicable to this product.

NCBI GI #

223942069

NCBI GeneID

5651

NCBI Accession #

NP_002763.2

UniProt Primary Accession #

P98073

UniProt Secondary Accession #

Q2NKL7

UniProt Related Accession #

P98073

GenBank Nucleotide #

NM_002772.2

NCBI Official Full Name

enteropeptidase

NCBI Official Synonym Full Names

transmembrane protease, serine 15

NCBI Official Symbol

TMPRSS15

NCBI Official Synonym Symbols

ENTK; PRSS7

UniProt Protein Name

Enteropeptidase

UniProt Synonym Protein Names

Enterokinase; Serine protease 7; Transmembrane protease serine 15Cleaved into the following 2 chains:Enteropeptidase non-catalytic heavy chain; Enteropeptidase catalytic light chain

UniProt Gene Name

TMPRSS15

UniProt Synonym Gene Names

ENTK; PRSS7

UniProt Entry Name

ENTK_HUMAN

NCBI Protein Information

enteropeptidase; serine protease 7; enterokinase catalytic subunit; protease, serine, 7 (enterokinase)

NCBI Summary for PRSS7

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]

UniProt Comments for PRSS7

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.9; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: integral to membrane; brush border

Molecular Function: serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; proteolysis

Disease: Enterokinase Deficiency

Research Articles on PRSS7

1. The Y174R variant showed improved specificities for substrates containing the sequences DDDDK (kcat/KM = 6.83 x 106 M-1 sec-1) and DDDDR (kcat/KM = 1.89 x 107 M-1 sec-1) relative to all other enteropeptidase variants reported to date.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.