Procollagen 1 C-Terminal Pro (PICP) ELISA Kit for Rat

Name: Rat PICP ELISA Kit; Procollagen 1 C-Terminal Pro; MyBioSource
Brand: MyBioSource
SKU: MBS092084
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS092084

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PICP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat PICP antibody to coat Microelisa Stripplate wells to… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Procollagen I C-Terminal Propeptide (PICP), ELISA Kit

Full Product Name

Rat Procollagen I C-Terminal Propeptide ELISA Kit

Product Gene Name

PICP elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

1.56ng/ml-50ng/ml.

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between Rat PICP and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

47,972 Da

OMIM

600270

3D Structure

ModBase 3D Structure for Q15113

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PICP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat PICP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PICP and PICP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PICP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of PICP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PICP purchase

MBS092084 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen I C-Terminal Propeptide (PICP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PICP. The ELISA analytical biochemical technique of the MBS092084 kit is based on PICP antibody-PICP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PICP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PICP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PICP. It may not necessarily be applicable to this product.

NCBI GI #

157653329

NCBI GeneID

5118

NCBI Accession #

NP_002584.2

UniProt Primary Accession #

Q15113

UniProt Secondary Accession #

O14550; B2R9E1

UniProt Related Accession #

Q15113

GenBank Nucleotide #

NM_002593.3

NCBI Official Full Name

procollagen C-endopeptidase enhancer 1

NCBI Official Synonym Full Names

procollagen C-endopeptidase enhancer

NCBI Official Symbol

PCOLCE

NCBI Official Synonym Symbols

PCPE; PCPE1; PCPE-1

UniProt Protein Name

Procollagen C-endopeptidase enhancer 1

UniProt Synonym Protein Names

Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer

UniProt Gene Name

PCOLCE

UniProt Synonym Gene Names

PCPE1; PCPE-1

UniProt Entry Name

PCOC1_HUMAN

NCBI Protein Information

procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer; procollagen, type 1, COOH-terminal proteinase enhancer

NCBI Summary for PICP

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

UniProt Comments for PICP

PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular matrix; extracellular space

Molecular Function: collagen binding; heparin binding; protein binding; protease activator activity

Biological Process: multicellular organismal development; proteolysis

Research Articles on PICP

1. The analysis of the PCPE-1 interaction network based on Gene Ontology terms suggests that, besides its role in collagen deposition, PCPE-1 might be involved in tumour growth, neurodegenerative diseases and angiogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PICP elisa kit

Reference Product	PubMed Publications
BMP1 elisa kit	>17 publications with PICP and BMP1
COL1A2 elisa kit	>2 publications with PICP and COL1A2
ADAMTS14 elisa kit	>1 publications with PICP and ADAMTS14
ADAMTS2 elisa kit	>1 publications with PICP and ADAMTS2

Pathways associated with PICP elisa kit

Products by Pathway	Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Crosslinking Of Collagen Fibrils Pathway antibodies	Crosslinking Of Collagen Fibrils Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with PICP elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>5 publications with PICP and Fibrosis
Neoplasms Antibodies	>4 publications with PICP and Neoplasms
Nervous System Diseases Antibodies	>3 publications with PICP and Nervous System Diseases
Cardiovascular Diseases Antibodies	>2 publications with PICP and Cardiovascular Diseases
Heart Diseases Antibodies	>2 publications with PICP and Heart Diseases
Necrosis Antibodies	>2 publications with PICP and Necrosis
Liver Diseases Antibodies	>1 publications with PICP and Liver Diseases
Breast Neoplasms Antibodies	>1 publications with PICP and Breast Neoplasms
Inflammation Antibodies	>1 publications with PICP and Inflammation
Hyperplasia Antibodies	>1 publications with PICP and Hyperplasia

Organs/Tissues associated with PICP elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>30 publications with PICP and Bone
Muscle Antibodies	>8 publications with PICP and Muscle
Heart Antibodies	>6 publications with PICP and Heart
Connective Tissue Antibodies	>6 publications with PICP and Connective Tissue
Embryonic Tissue Antibodies	>4 publications with PICP and Embryonic Tissue
Eye Antibodies	>3 publications with PICP and Eye
Liver Antibodies	>3 publications with PICP and Liver
Brain Antibodies	>3 publications with PICP and Brain
Kidney Antibodies	>2 publications with PICP and Kidney
Uterus Antibodies	>2 publications with PICP and Uterus