Cadherin 5, Type 2 (Vascular Endothelium) (CDH5) ELISA Kit for Mouse

Name: Mouse CDH5 ELISA Kit; Cadherin 5, Type 2 (Vascular Endothelium); MyBioSource
Brand: MyBioSource
SKU: MBS921276
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS921276

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDH5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDH5 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

cadherin 5, type 2 (vascular endothelium) (CDH5), ELISA Kit

Full Product Name

Mouse Cadherin-5, CDH5 ELISA Kit

Product Gene Name

CDH5 elisa kit

Product Synonym Names

Mouse Cadherin-5 (CDH5) ELISA kit; 7B4; CD144; FLJ17376; 7B4 antigen; VE-cadherin; cadherin 5; type 2; cadherin 5; type 2; VE-cadherin (vascular epithelium) ; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5; type 2 (vascular endothelium)

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.235 ng/ml-15 ng/ml

Sensitivity

0.058 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CDH5. No significant cross-reactivity or interference between Mouse CDH5 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

87,903 Da

3D Structure

ModBase 3D Structure for P55284

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Cell Adhesion

Protein Biological Process 3

Cell adhesion

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CDH5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDH5 purchase

MBS921276 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cadherin 5, type 2 (vascular endothelium) (CDH5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDH5. The ELISA analytical biochemical technique of the MBS921276 kit is based on CDH5 antibody-CDH5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CDH5. It may not necessarily be applicable to this product.

NCBI GI #

38490561

NCBI GeneID

12562

NCBI Accession #

NP_033998.2

UniProt Primary Accession #

P55284

UniProt Secondary Accession #

O35542

UniProt Related Accession #

P55284

GenBank Nucleotide #

NM_009868.4

NCBI Official Full Name

cadherin-5

NCBI Official Synonym Full Names

cadherin 5

NCBI Official Symbol

Cdh5

NCBI Official Synonym Symbols

7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225

UniProt Protein Name

Cadherin-5

UniProt Synonym Protein Names

Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144

Protein Family

Cadherin

UniProt Gene Name

Cdh5

UniProt Synonym Gene Names

VE-cadherin

UniProt Entry Name

CADH5_MOUSE

NCBI Protein Information

cadherin-5; VE-cadherin; 7B4/cadherin-5; vascular endothelial cadherin

UniProt Comments for CDH5

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: tight junction; adherens junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; metal ion binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell adhesion; cell adhesion; homophilic cell adhesion; blood vessel maturation

Research Articles on CDH5

1. Fusing VE-cadherin to alpha-catenin presents a mutation, which leads to embryonic lethality, due to a lack of fetal liver hematopoiesis and severe lymphedema but no detectable defects in blood vessel formation and remodeling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDH5 elisa kit

Reference Product	PubMed Publications
Ctnnb1 elisa kit	>1 publications with CDH5 and Ctnnb1

Pathways associated with CDH5 elisa kit

Products by Pathway	Pathway Diagram
Adherens Junctions Interactions Pathway antibodies	Adherens Junctions Interactions Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Junction Organization Pathway antibodies	Cell Junction Organization Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Cell-cell Junction Organization Pathway antibodies	Cell-cell Junction Organization Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with CDH5 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>5 publications with CDH5 and Cardiovascular Diseases
Breast Neoplasms Antibodies	>3 publications with CDH5 and Breast Neoplasms
Necrosis Antibodies	>2 publications with CDH5 and Necrosis
Inflammation Antibodies	>2 publications with CDH5 and Inflammation
Lung Neoplasms Antibodies	>2 publications with CDH5 and Lung Neoplasms
Adenocarcinoma Antibodies	>2 publications with CDH5 and Adenocarcinoma
Myocardial Infarction Antibodies	>2 publications with CDH5 and Myocardial Infarction
Colorectal Neoplasms Antibodies	>2 publications with CDH5 and Colorectal Neoplasms
Atherosclerosis Antibodies	>2 publications with CDH5 and Atherosclerosis
Chromosome Aberrations Antibodies	>1 publications with CDH5 and Chromosome Aberrations

Organs/Tissues associated with CDH5 elisa kit

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>9 publications with CDH5 and Other
Embryonic Tissue Antibodies	>9 publications with CDH5 and Embryonic Tissue
Heart Antibodies	>4 publications with CDH5 and Heart
Kidney Antibodies	>3 publications with CDH5 and Kidney
Brain Antibodies	>3 publications with CDH5 and Brain
Lung Antibodies	>2 publications with CDH5 and Lung
Mammary Gland Antibodies	>1 publications with CDH5 and Mammary Gland