Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) ELISA Kit for Human

Name: Human ROBO1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS921385
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS921385

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROBO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROBO1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), ELISA Kit

Full Product Name

Human Roundabout homolog 1, ROBO1 ELISA Kit

Product Gene Name

ROBO1 elisa kit

Product Synonym Names

Human Roundabout homolog 1 (ROBO1) ELISA kit; DUTT1; FLJ21882; MGC131599; MGC133277; SAX3; roundabout 1; roundabout; axon guidance receptor; homolog 1 (Drosophila)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.156 ng/ml-10 ng/ml

Sensitivity

< 0.039 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human ROBO1. No significant cross-reactivity or interference between human ROBO1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

180,930 Da

OMIM

602430

3D Structure

ModBase 3D Structure for Q9Y6N7

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ROBO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROBO1 purchase

MBS921385 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROBO1. The ELISA analytical biochemical technique of the MBS921385 kit is based on ROBO1 antibody-ROBO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROBO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROBO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ROBO1. It may not necessarily be applicable to this product.

NCBI GI #

224994173

NCBI GeneID

6091

NCBI Accession #

NP_001139317.1

UniProt Primary Accession #

Q9Y6N7

UniProt Secondary Accession #

Q1RMC7; Q7Z300; Q9BUS7; B2RXI1; D3DU36; E9PD49

UniProt Related Accession #

Q9Y6N7

GenBank Nucleotide #

NM_001145845.1

NCBI Official Full Name

roundabout homolog 1 isoform d

NCBI Official Synonym Full Names

roundabout, axon guidance receptor, homolog 1 (Drosophila)

NCBI Official Symbol

ROBO1

NCBI Official Synonym Symbols

SAX3; DUTT1

UniProt Protein Name

Roundabout homolog 1

UniProt Synonym Protein Names

Deleted in U twenty twenty; H-Robo-1

Protein Family

Roundabout

UniProt Gene Name

ROBO1

UniProt Synonym Gene Names

DUTT1

UniProt Entry Name

ROBO1_HUMAN

NCBI Protein Information

roundabout homolog 1; deleted in U twenty twenty

NCBI Summary for ROBO1

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

UniProt Comments for ROBO1

ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p12

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; axolemma

Molecular Function: identical protein binding; protein binding; axon guidance receptor activity; LRR domain binding

Biological Process: caspase activation; nervous system development; axon guidance; negative regulation of negative chemotaxis; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; positive regulation of axonogenesis; cell adhesion; homophilic cell adhesion; axon midline choice point recognition; negative regulation of mammary gland epithelial cell proliferation

Research Articles on ROBO1

1. Full-length Robo1 is present almost exclusively as a dimer; parallel studies demonstrate the biological activity of Slit2 and its interaction with Robo1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ROBO1 elisa kit

Reference Product	PubMed Publications
SLIT2 elisa kit	>79 publications with ROBO1 and SLIT2
ROBO2 elisa kit	>65 publications with ROBO1 and ROBO2
SLIT1 elisa kit	>49 publications with ROBO1 and SLIT1
SRGAP1 elisa kit	>3 publications with ROBO1 and SRGAP1

Pathways associated with ROBO1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Rac Pathway antibodies	Activation Of Rac Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Inactivation Of Cdc42 And Rac Pathway antibodies	Inactivation Of Cdc42 And Rac Pathway Diagram
Netrin-1 Signaling Pathway antibodies	Netrin-1 Signaling Pathway Diagram
Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies	Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
Role Of Abl In Robo-Slit Signaling Pathway antibodies	Role Of Abl In Robo-Slit Signaling Pathway Diagram
Signaling By Robo Receptor Pathway antibodies	Signaling By Robo Receptor Pathway Diagram

Diseases associated with ROBO1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>47 publications with ROBO1 and Neoplasms
Carcinoma Antibodies	>30 publications with ROBO1 and Carcinoma
Nervous System Diseases Antibodies	>23 publications with ROBO1 and Nervous System Diseases
Learning Disorders Antibodies	>16 publications with ROBO1 and Learning Disorders
Neurobehavioral Manifestations Antibodies	>16 publications with ROBO1 and Neurobehavioral Manifestations
Breast Neoplasms Antibodies	>12 publications with ROBO1 and Breast Neoplasms
Lung Diseases Antibodies	>11 publications with ROBO1 and Lung Diseases
Adenocarcinoma Antibodies	>10 publications with ROBO1 and Adenocarcinoma
Lung Neoplasms Antibodies	>9 publications with ROBO1 and Lung Neoplasms
Neoplasm Invasiveness Antibodies	>8 publications with ROBO1 and Neoplasm Invasiveness

Organs/Tissues associated with ROBO1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>61 publications with ROBO1 and Brain
Embryonic Tissue Antibodies	>43 publications with ROBO1 and Embryonic Tissue
Vascular Antibodies	>25 publications with ROBO1 and Vascular
Blood Antibodies	>20 publications with ROBO1 and Blood
Eye Antibodies	>19 publications with ROBO1 and Eye
Lung Antibodies	>15 publications with ROBO1 and Lung
Muscle Antibodies	>8 publications with ROBO1 and Muscle
Liver Antibodies	>4 publications with ROBO1 and Liver
Mammary Gland Antibodies	>4 publications with ROBO1 and Mammary Gland
Ovary Antibodies	>4 publications with ROBO1 and Ovary