Human DNAJC19 ELISA Kit – Cat# MBS921712

Dnaj (Hsp40) Homolog, Subfamily C, Member 19 (DNAJC19) ELISA Kit for Human

Name: Human DNAJC19 ELISA Kit; Dnaj (Hsp40) Homolog, Subfamily C, Member 19; MyBioSource
Brand: MyBioSource
SKU: MBS921712
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS921712

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DNAJC19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAJC19 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

DnaJ (Hsp40) homolog, subfamily C, member 19 (DNAJC19), ELISA Kit

Full Product Name

Human Mitochondrial import inner membrane translocase subunit TIM14, DNAJC19 ELISA Kit

Product Gene Name

DNAJC19 elisa kit

Product Synonym Names

Human Mitochondrial import inner membrane translocase subunit TIM14 (DNAJC19) ELISA kit; TIM14; TIMM14; DnaJ homolog; subfamily C; member 19; homolog of yeast TIM14; DnaJ (Hsp40) homolog; subfamily C; member 19

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

28 pg/ml -1800 pg/ml.

Sensitivity

The minimum detectable dose of human DNAJC19 is typically less than 7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human DNAJC19. No significant cross-reactivity or interference between human DNAJC19 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

12,499 Da

OMIM

gene 610198

3D Structure

ModBase 3D Structure for Q96DA6

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DNAJC19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNAJC19 purchase

MBS921712 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DnaJ (Hsp40) homolog, subfamily C, member 19 (DNAJC19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DNAJC19. The ELISA analytical biochemical technique of the MBS921712 kit is based on DNAJC19 antibody-DNAJC19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNAJC19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNAJC19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DNAJC19. It may not necessarily be applicable to this product.

NCBI GI #

298231213

NCBI GeneID

131118

NCBI Accession #

NP_001177162.1

UniProt Primary Accession #

Q96DA6

UniProt Secondary Accession #

B2R4B1; C9JBV1

UniProt Related Accession #

Q96DA6

GenBank Nucleotide #

NM_001190233.1

NCBI Official Full Name

mitochondrial import inner membrane translocase subunit TIM14 isoform 2

NCBI Official Synonym Full Names

DnaJ (Hsp40) homolog, subfamily C, member 19

NCBI Official Symbol

DNAJC19

NCBI Official Synonym Symbols

PAM18; TIM14; TIMM14

UniProt Protein Name

Mitochondrial import inner membrane translocase subunit TIM14

UniProt Synonym Protein Names

DnaJ homolog subfamily C member 19

UniProt Gene Name

DNAJC19

UniProt Synonym Gene Names

TIM14; TIMM14

UniProt Entry Name

TIM14_HUMAN

NCBI Protein Information

mitochondrial import inner membrane translocase subunit TIM14; homolog of yeast TIM14

NCBI Summary for DNAJC19

The protein encoded by this gene is thought to be part of a complex involved in the ATP-dependent transport of transit peptide-containing proteins from the inner cell membrane to the mitochondrial matrix. Defects in this gene are a cause of 3-methylglutaconic aciduria type 5 (MGA5), also known as dilated cardiomyopathy with ataxia (DCMA). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 2, 6, 10, 14 and 19. [provided by RefSeq, Jan 2012]

UniProt Comments for DNAJC19

DNAJC19: Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity. Defects in DNAJC19 are the cause of 3-methylglutaconic aciduria type 5 (MGA5); also known as dilated cardiomyopathy with ataxia (DCMA). MGA5 is an autosomal recessive disorder characterized by early-onset dilated cardiomyopathy, growth failure, cerebellar ataxia causing significant motor delays, testicular dysgenesis, growth failure, and significant increases in urine organic acids, particularly 3-methylglutaconic acid and 3-methylglutaric acid. Belongs to the TIM14 family.

Protein type: Chaperone; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: protein complex; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: protein binding

Biological Process: genitalia development; cellular protein metabolic process; visual perception; protein folding; protein targeting to mitochondrion

Disease: 3-methylglutaconic Aciduria, Type V

Research Articles on DNAJC19

1. A report of a new mutation in the human DNAJC19 gene that causes early onset dilated cardiomyopathy syndrome in two brothers of Finnish origin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DNAJC19 elisa kit

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Mitochondrial Protein Import Pathway antibodies	Mitochondrial Protein Import Pathway Diagram

Diseases associated with DNAJC19 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>3 publications with DNAJC19 and Cardiovascular Diseases

Organs/Tissues associated with DNAJC19 elisa kit

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>1 publications with DNAJC19 and Prostate