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Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS922741

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOC3 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
amine oxidase, copper containing 3 (vascular adhesion protein 1) (AOC3), ELISA Kit
Full Product Name
Rat Membrane primary amine oxidase, AOC3 ELISA Kit
Product Gene Name
AOC3 elisa kit
Product Synonym Names
Rat Membrane primary amine oxidase (AOC3) ELISA kit; HPAO; SSAO; VAP-1; VAP1; amine oxidase; copper containing 3; copper amine oxidase; semicarbazide-sensitive amine oxidase; vascular adhesion protein 1; amine oxidase; copper containing 3 (vascular adhesion protein 1)
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
7.8 ng/ml -500 ng/ml.
Sensitivity
The minimum detectable dose of rat AOC3 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of rat AOC3. No significant cross-reactivity or interference between rat AOC3 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
84,981 Da
3D Structure
ModBase 3D Structure for O08590
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of AOC3 elisa kit

Rat AOC3 ELISA Kit – Cat# MBS922741

Other Notes

Small volumes of AOC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AOC3 purchase

MBS922741 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amine oxidase, copper containing 3 (vascular adhesion protein 1) (AOC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AOC3. The ELISA analytical biochemical technique of the MBS922741 kit is based on AOC3 antibody-AOC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AOC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AOC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AOC3. It may not necessarily be applicable to this product.

NCBI GI #
71896590
NCBI GeneID
29473
NCBI Accession #
NP_113770.2
UniProt Primary Accession #
O08590
UniProt Secondary Accession #
Q497D2; Q5R1T5
UniProt Related Accession #
O08590
GenBank Nucleotide #
NM_031582.2
NCBI Official Full Name
membrane primary amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper containing 3
NCBI Official Symbol
Aoc3
NCBI Official Synonym Symbols
SSAO
UniProt Protein Name
Membrane primary amine oxidase
UniProt Synonym Protein Names
Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1
Protein Family
Allene oxide cyclase
UniProt Gene Name
Aoc3
UniProt Synonym Gene Names
SSAO; VAP-1
UniProt Entry Name
AOC3_RAT
NCBI Protein Information
membrane primary amine oxidase; VP97; VAP-1; copper amine oxidase; vascular adhesion protein 1; semicarbazide-sensitive amine oxidase

NCBI Summary for AOC3

localized to the adipocyte plasma membrane and to glucose transporter Glut4 containing vesicles [RGD, Feb 2006]

UniProt Comments for AOC3

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Cell surface; Calcium-binding; Membrane protein, integral; Oxidoreductase; Cell adhesion; Motility/polarity/chemotaxis; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - phenylalanine; EC 1.4.3.21; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine

Cellular Component: extracellular space; cell surface; microvillus; intracellular membrane-bound organelle; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; eating behavior; positive regulation of acute inflammatory response; positive regulation of leukocyte migration; regulation of blood pressure; amine metabolic process; cell adhesion; cation transport; leukocyte migration during inflammatory response; aging
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Research Articles on AOC3

1. The changes of K(M) and k(c)/K(M) values of copper amine oxidase appear due to the presence of positively charged groups in the substrate molecules.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AOC3 elisa kit

 Products by Pathway  Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Beta-Alanine Metabolism Pathway antibodies  Beta-Alanine Metabolism Pathway Diagram
 Beta-Alanine Metabolism Pathway antibodies  Beta-Alanine Metabolism Pathway Diagram

Diseases associated with AOC3 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >63 publications with AOC3 and Inflammation
 Neoplasms Antibodies  >15 publications with AOC3 and Neoplasms
 Necrosis Antibodies  >10 publications with AOC3 and Necrosis
 Central Nervous System Diseases Antibodies  >10 publications with AOC3 and Central Nervous System Diseases
 Atherosclerosis Antibodies  >10 publications with AOC3 and Atherosclerosis
 Liver Neoplasms Antibodies  >7 publications with AOC3 and Liver Neoplasms
 Adenocarcinoma Antibodies  >6 publications with AOC3 and Adenocarcinoma
 Gastroenteritis Antibodies  >5 publications with AOC3 and Gastroenteritis
 Liver Cirrhosis Antibodies  >5 publications with AOC3 and Liver Cirrhosis
 Hyperglycemia Antibodies  >5 publications with AOC3 and Hyperglycemia

Organs/Tissues associated with AOC3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >17 publications with AOC3 and Muscle
 Kidney Antibodies  >10 publications with AOC3 and Kidney
 Heart Antibodies  >10 publications with AOC3 and Heart
 Adipose Tissue Antibodies  >7 publications with AOC3 and Adipose Tissue
 Eye Antibodies  >4 publications with AOC3 and Eye
 Lung Antibodies  >3 publications with AOC3 and Lung
 Ovary Antibodies  >2 publications with AOC3 and Ovary
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