Allograft Inflammatory Factor 1 (AIF1) ELISA Kit for Mouse

Name: Mouse AIF1 ELISA Kit; Allograft Inflammatory Factor 1; MyBioSource
Brand: MyBioSource
SKU: MBS922753
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS922753

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AIF1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

allograft inflammatory factor 1 (AIF1), ELISA Kit

Full Product Name

Mouse Allograft inflammatory factor 1, AIF1 ELISA Kit

Product Gene Name

AIF1 elisa kit

Product Synonym Names

Mouse Allograft inflammatory factor 1 (AIF1) ELISA kit; DADB-70P7.8; AIF-1; IBA1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; allograft inflammatory factor 1

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

1.10 ng/ml-800 ng/ml

Sensitivity

< 1.10 ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse AIF1. No significant cross-reactivity or interference between mouse AIF1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

16,911 Da

3D Structure

ModBase 3D Structure for O70200

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of AIF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AIF1 purchase

MBS922753 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the allograft inflammatory factor 1 (AIF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AIF1. The ELISA analytical biochemical technique of the MBS922753 kit is based on AIF1 antibody-AIF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AIF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AIF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AIF1. It may not necessarily be applicable to this product.

NCBI GI #

9506379

NCBI GeneID

11629

NCBI Accession #

NP_062340.1

UniProt Primary Accession #

O70200

UniProt Related Accession #

O70200

GenBank Nucleotide #

NM_019467.2

NCBI Official Full Name

allograft inflammatory factor 1

NCBI Official Synonym Full Names

allograft inflammatory factor 1

NCBI Official Symbol

Aif1

NCBI Official Synonym Symbols

G1; Iba1; AIF-1; AI607846; D17H6S50E

UniProt Protein Name

Allograft inflammatory factor 1

UniProt Synonym Protein Names

Ionized calcium-binding adapter molecule 1

Protein Family

Allograft inflammatory factor

UniProt Gene Name

Aif1

UniProt Synonym Gene Names

Iba1; AIF-1

UniProt Entry Name

AIF1_MOUSE

NCBI Protein Information

allograft inflammatory factor 1; testis specific; ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule 1

UniProt Comments for AIF1

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: actin filament; perikaryon; cytosol; ruffle; cell projection; cytoskeleton; membrane; lamellipodium; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; nucleus; phagocytic cup

Molecular Function: actin filament binding; protein binding; metal ion binding; calcium ion binding; actin binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; actin filament polymerization; positive regulation of smooth muscle cell proliferation; positive regulation of muscle hyperplasia; microglial cell activation; Rac protein signal transduction; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; positive regulation of cell migration; negative regulation of apoptosis

Research Articles on AIF1

1. These results imply daintain/AIF-1 triggers type 1 diabetes probably via arousing immune cells activation and induction of NO production in pancreas of NOD mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with AIF1 elisa kit

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>148 publications with AIF1 and Disease Models, Animal
Inflammation Antibodies	>84 publications with AIF1 and Inflammation
Cardiovascular Diseases Antibodies	>69 publications with AIF1 and Cardiovascular Diseases
Necrosis Antibodies	>54 publications with AIF1 and Necrosis
Ischemia Antibodies	>45 publications with AIF1 and Ischemia
Pain Antibodies	>38 publications with AIF1 and Pain
Nerve Degeneration Antibodies	>28 publications with AIF1 and Nerve Degeneration
Skin Diseases Antibodies	>20 publications with AIF1 and Skin Diseases
Neoplasms Antibodies	>18 publications with AIF1 and Neoplasms
Drug Toxicity Antibodies	>17 publications with AIF1 and Drug Toxicity

Organs/Tissues associated with AIF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>232 publications with AIF1 and Brain
Bone Marrow Antibodies	>21 publications with AIF1 and Bone Marrow
Eye Antibodies	>16 publications with AIF1 and Eye
Embryonic Tissue Antibodies	>12 publications with AIF1 and Embryonic Tissue
Liver Antibodies	>10 publications with AIF1 and Liver
Spleen Antibodies	>8 publications with AIF1 and Spleen
Kidney Antibodies	>7 publications with AIF1 and Kidney
Lung Antibodies	>6 publications with AIF1 and Lung
Uncharacterized Tissue Antibodies	>5 publications with AIF1 and Uncharacterized Tissue
Pancreas Antibodies	>4 publications with AIF1 and Pancreas