PTK2 Protein Tyrosine Kinase 2 (PTK2) ELISA Kit for Chicken

Name: Chicken PTK2 ELISA Kit; PTK2 Protein Tyrosine Kinase 2; MyBioSource
Brand: MyBioSource
SKU: MBS922799
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS922799

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for PTK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a biotin-conjugated PTK2. A… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

PTK2 protein tyrosine kinase 2, ELISA Kit

Full Product Name

Chicken Focal adhesion kinase 1, PTK2 ELISA Kit

Product Gene Name

PTK2 elisa kit

Product Synonym Names

Chicken Focal adhesion kinase 1 (PTK2) ELISA kit; FADK; FAK; FAK1; FRNK; pp125FAK; FAK-related non-kinase polypeptide; focal adhesion kinase 1; PTK2 protein tyrosine kinase 2

Species Reactivity

Chicken

Assay Type

Competitive

Specificity

This assay has high sensitivity and excellent specificity for detection of Chicken PTK2. No significant cross-reactivity or interference between Chicken PTK2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

119,207 Da

3D Structure

ModBase 3D Structure for Q00944

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PTK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTK2 purchase

MBS922799 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PTK2 protein tyrosine kinase 2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTK2. The ELISA analytical biochemical technique of the MBS922799 kit is based on PTK2 antibody-PTK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PTK2. It may not necessarily be applicable to this product.

NCBI GI #

45382167

NCBI GeneID

396416

NCBI Accession #

NP_990766.1

UniProt Primary Accession #

Q00944

UniProt Related Accession #

Q00944

GenBank Nucleotide #

NM_205435.1

NCBI Official Full Name

focal adhesion kinase 1

NCBI Official Synonym Full Names

protein tyrosine kinase 2

NCBI Official Symbol

PTK2

NCBI Official Synonym Symbols

FAK; FRNK; p125FAK

UniProt Protein Name

Focal adhesion kinase 1

UniProt Synonym Protein Names

Focal adhesion kinase-related nonkinase; FRNK; p41/p43FRNK; Protein-tyrosine kinase 2; p125FAK; pp125FAK

Protein Family

Focal adhesion kinase

UniProt Gene Name

PTK2

UniProt Synonym Gene Names

FAK; FAK1; FADK 1; FRNK; p41/p43FRNK

UniProt Entry Name

FAK1_CHICK

NCBI Protein Information

focal adhesion kinase 1; FADK 1; pp125FAK; p41/p43FRNK; protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; focal adhesion kinase-related nonkinase

UniProt Comments for PTK2

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Fak family

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; perinuclear region of cytoplasm; microtubule organizing center; nucleus

Molecular Function: identical protein binding; protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; peptidyl-tyrosine phosphorylation; signal complex assembly; innate immune response; cytoskeleton organization and biogenesis; angiogenesis; cell differentiation; regulation of cell proliferation; negative regulation of apoptosis

Research Articles on PTK2

1. findings uncover an inhibitory interaction between the FAK FERM domain and sarcomeric myosin that presents potential opportunities to modulate the cardiac hypertrophic response through changes in FAK activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PTK2 elisa kit

Reference Product	PubMed Publications
ITGB5 elisa kit	>1 publications with PTK2 and ITGB5

Pathways associated with PTK2 elisa kit

Products by Pathway	Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
VEGF Signaling Pathway antibodies	VEGF Signaling Pathway Diagram
VEGF Signaling Pathway antibodies	VEGF Signaling Pathway Diagram

Diseases associated with PTK2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>700 publications with PTK2 and Neoplasms
Carcinoma Antibodies	>280 publications with PTK2 and Carcinoma
Neoplasm Metastasis Antibodies	>135 publications with PTK2 and Neoplasm Metastasis
Adenocarcinoma Antibodies	>107 publications with PTK2 and Adenocarcinoma
Breast Neoplasms Antibodies	>104 publications with PTK2 and Breast Neoplasms
Disease Models, Animal Antibodies	>76 publications with PTK2 and Disease Models, Animal
Lung Diseases Antibodies	>70 publications with PTK2 and Lung Diseases
Neoplasms, Experimental Antibodies	>70 publications with PTK2 and Neoplasms, Experimental
Intestinal Diseases Antibodies	>69 publications with PTK2 and Intestinal Diseases
Lung Neoplasms Antibodies	>67 publications with PTK2 and Lung Neoplasms

Organs/Tissues associated with PTK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>348 publications with PTK2 and Other
Muscle Antibodies	>215 publications with PTK2 and Muscle
Brain Antibodies	>149 publications with PTK2 and Brain
Liver Antibodies	>97 publications with PTK2 and Liver
Embryonic Tissue Antibodies	>92 publications with PTK2 and Embryonic Tissue
Heart Antibodies	>78 publications with PTK2 and Heart
Ovary Antibodies	>30 publications with PTK2 and Ovary
Head Antibodies	>22 publications with PTK2 and Head
Testis Antibodies	>8 publications with PTK2 and Testis
Small Intestine Antibodies	>5 publications with PTK2 and Small Intestine