Sperm Adhesion Molecule 1 (Ph-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) ELISA Kit for Human

Name: Human SPAM1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS924065
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS924065

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPAM1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), ELISA Kit

Full Product Name

Human Hyaluronidase PH-20, SPAM1 ELISA Kit

Product Gene Name

SPAM1 elisa kit

Product Synonym Names

Human Hyaluronidase PH-20 (SPAM1) ELISA kit; HYA1; HYAL1; HYAL3; HYAL5; MGC26532; PH-20; PH20; SPAG15; OTTHUMP00000211901; hyaluronoglucosaminidase; sperm adhesion molecule 1; sperm surface protein PH-20; sperm adhesion molecule 1 (PH-20 hyaluronidase; zona pellucida binding)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, urine

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

< 3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human SPAM1. No significant cross-reactivity or interference between human SPAM1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

57,848 Da

OMIM

600930

3D Structure

ModBase 3D Structure for P38567

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SPAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPAM1 purchase

MBS924065 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPAM1. The ELISA analytical biochemical technique of the MBS924065 kit is based on SPAM1 antibody-SPAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SPAM1. It may not necessarily be applicable to this product.

NCBI GI #

291290979

NCBI GeneID

6677

NCBI Accession #

NP_001167515.1

UniProt Primary Accession #

P38567

UniProt Secondary Accession #

Q8TC30

UniProt Related Accession #

P38567

GenBank Nucleotide #

NM_001174044.1

NCBI Official Full Name

hyaluronidase PH-20 isoform 2

NCBI Official Synonym Full Names

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

NCBI Official Symbol

SPAM1

NCBI Official Synonym Symbols

HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n

UniProt Protein Name

Hyaluronidase PH-20

UniProt Synonym Protein Names

Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20

Protein Family

Hyaluronidase

UniProt Gene Name

SPAM1

UniProt Synonym Gene Names

HYAL3; PH20; Hyal-PH20

UniProt Entry Name

HYALP_HUMAN

NCBI Protein Information

hyaluronidase PH-20; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; epididymis secretory sperm binding protein Li 96n

NCBI Summary for SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

UniProt Comments for SPAM1

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Cell surface; Membrane protein, GPI anchor; Hydrolase

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction

Research Articles on SPAM1

1. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SPAM1 elisa kit

Products by Pathway	Pathway Diagram
Chondroitin Sulfate Degradation Pathway antibodies	Chondroitin Sulfate Degradation Pathway Diagram
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Dermatan Sulfate Degradation Pathway antibodies	Dermatan Sulfate Degradation Pathway Diagram
Dermatan Sulfate Degradation Pathway antibodies	Dermatan Sulfate Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram

Diseases associated with SPAM1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with SPAM1 and Neoplasms
Kidney Diseases Antibodies	>1 publications with SPAM1 and Kidney Diseases
Necrosis Antibodies	>1 publications with SPAM1 and Necrosis
Liver Neoplasms Antibodies	>1 publications with SPAM1 and Liver Neoplasms

Organs/Tissues associated with SPAM1 elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>25 publications with SPAM1 and Testis