Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit for Rat

Name: Rat AMY2 ELISA Kit; Amylase Alpha 2, Pancreatic; MyBioSource
Brand: MyBioSource
SKU: MBS009251
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS009251

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AMY2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMY2 antibody to coat Microelisa Stripplate wells to… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Amylase Alpha 2, Pancreatic (AMY2), ELISA Kit

Full Product Name

Rat Amylase Alpha 2, Pancreatic ELISA Kit

Product Gene Name

AMY2 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

6.25pg/ml-200pg/ml.

Sensitivity

1.0pg/ml.

Specificity

No significant cross-reactivity or interference between Rat AMY2 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

57,707 Da

OMIM

104650

3D Structure

ModBase 3D Structure for P04746

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated AMY2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMY2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AMY2 and AMY2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMY2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of AMY2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMY2 purchase

MBS009251 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amylase Alpha 2, Pancreatic (AMY2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMY2. The ELISA analytical biochemical technique of the MBS009251 kit is based on AMY2 antibody-AMY2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMY2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMY2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AMY2. It may not necessarily be applicable to this product.

NCBI GI #

529397

NCBI GeneID

279

NCBI Accession #

AAA51724.1

UniProt Primary Accession #

P04746

UniProt Secondary Accession #

Q9UBH3

UniProt Related Accession #

P04746

NCBI Official Full Name

amylase

NCBI Official Synonym Full Names

amylase, alpha 2A (pancreatic)

NCBI Official Symbol

AMY2A

NCBI Official Synonym Symbols

PA; AMY2; AMY2B

UniProt Protein Name

Pancreatic alpha-amylase

UniProt Synonym Protein Names

1,4-alpha-D-glucan glucanohydrolase

Protein Family

Pancreatic alpha-amylase

UniProt Gene Name

AMY2A

UniProt Synonym Gene Names

UniProt Entry Name

AMYP_HUMAN

NCBI Protein Information

pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase

NCBI Summary for AMY2

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

UniProt Comments for AMY2

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - starch and sucrose; Hydrolase; Secreted; EC 3.2.1.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

Research Articles on AMY2

1. Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AMY2 elisa kit

Products by Pathway	Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Digestion Of Dietary Carbohydrate Pathway antibodies	Digestion Of Dietary Carbohydrate Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
Myoclonic Epilepsy Of Lafora Pathway antibodies	Myoclonic Epilepsy Of Lafora Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram

Diseases associated with AMY2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with AMY2 and Neoplasms
Inflammation Antibodies	>2 publications with AMY2 and Inflammation
Congenital Abnormalities Antibodies	>1 publications with AMY2 and Congenital Abnormalities

Organs/Tissues associated with AMY2 elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>5 publications with AMY2 and Pancreas
Bladder Antibodies	>1 publications with AMY2 and Bladder
Salivary Gland Antibodies	>1 publications with AMY2 and Salivary Gland