Chemokine (C-C Motif) Receptor 2 (CCR2) ELISA Kit for Rat

Name: Rat CCR2 ELISA Kit; Chemokine (C-C Motif) Receptor 2; MyBioSource
Brand: MyBioSource
SKU: MBS925375
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS925375

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCR2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

chemokine (C-C motif) receptor 2 (CCR2), ELISA Kit

Full Product Name

Rat C-C chemokine receptor type 2, CCR2 ELISA Kit

Product Gene Name

CCR2 elisa kit

Product Synonym Names

Rat C-C chemokine receptor type 2 (CCR2) ELISA kit; CC-CKR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R; MCP-1 receptor; chemokine (C-C) receptor 2; monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor; chemokine (C-C motif) receptor 2

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat CCR2. No significant cross-reactivity or interference between Rat CCR2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

42,763 Da

3D Structure

ModBase 3D Structure for O55193

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CCR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCR2 purchase

MBS925375 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chemokine (C-C motif) receptor 2 (CCR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCR2. The ELISA analytical biochemical technique of the MBS925375 kit is based on CCR2 antibody-CCR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CCR2. It may not necessarily be applicable to this product.

NCBI GI #

11177914

NCBI GeneID

60463

NCBI Accession #

NP_068638.1

UniProt Primary Accession #

O55193

UniProt Related Accession #

O55193

GenBank Nucleotide #

NM_021866.1

NCBI Official Full Name

C-C chemokine receptor type 2

NCBI Official Synonym Full Names

chemokine (C-C motif) receptor 2

NCBI Official Symbol

Ccr2

UniProt Protein Name

C-C chemokine receptor type 2

UniProt Synonym Protein Names

CD_antigen: CD192

Protein Family

C-C chemokine receptor

UniProt Gene Name

Ccr2

UniProt Synonym Gene Names

Cmkbr2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2

UniProt Entry Name

CCR2_RAT

NCBI Protein Information

C-C chemokine receptor type 2; CCR-2; CC-CKR-2; C-C CKR-2; chemokine receptor CCR2

NCBI Summary for CCR2

mediates leukocyte migration and activation [RGD, Feb 2006]

UniProt Comments for CCR2

CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Cellular Component: cell soma; perinuclear region of cytoplasm; dendrite; cytoplasm; integral to membrane; plasma membrane; perikaryon; cytosol; external side of plasma membrane

Molecular Function: protein homodimerization activity; CCR2 chemokine receptor binding; C-C chemokine receptor activity; cytokine binding; chemokine binding; C-C chemokine binding

Biological Process: positive regulation of T-helper 1 type immune response; sensory perception of pain; regulation of cell migration; monocyte chemotaxis; positive regulation of MAPKKK cascade; hemopoiesis; cellular homeostasis; inflammatory response; positive regulation of tumor necrosis factor biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; negative regulation of angiogenesis; inflammatory response to antigenic stimulus; response to hyperoxia; negative regulation of T-helper 2 type immune response; positive regulation of interleukin-2 production; response to hypoxia; cellular defense response; positive regulation of alpha-beta T cell proliferation; immune response; blood vessel remodeling; positive regulation of T cell activation; negative regulation of eosinophil degranulation; positive regulation of inflammatory response

Research Articles on CCR2

1. investigated the expression changes and cellular localization of spinal CCR2 in a rat model of bone cancer induced by Walker 256 cell inoculation. expression of CCR2 in the spinal cord was significantly increased on day 6, 12, and 18 in bone cancer rats

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CCR2 elisa kit

Products by Pathway	Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram
Peptide GPCRs Pathway antibodies	Peptide GPCRs Pathway Diagram

Diseases associated with CCR2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>593 publications with CCR2 and Inflammation
Cardiovascular Diseases Antibodies	>273 publications with CCR2 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>264 publications with CCR2 and Disease Models, Animal
Vascular Diseases Antibodies	>262 publications with CCR2 and Vascular Diseases
Nervous System Diseases Antibodies	>220 publications with CCR2 and Nervous System Diseases
Necrosis Antibodies	>201 publications with CCR2 and Necrosis
Atherosclerosis Antibodies	>165 publications with CCR2 and Atherosclerosis
Lung Diseases Antibodies	>136 publications with CCR2 and Lung Diseases
Arteriosclerosis Antibodies	>112 publications with CCR2 and Arteriosclerosis
Kidney Diseases Antibodies	>89 publications with CCR2 and Kidney Diseases