Interferon-Induced Protein with Tetratricopeptide Repeats 1 (IFIT1) ELISA Kit for Human

Name: Human IFIT1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS925861
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS925861

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFIT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFIT1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), ELISA Kit

Full Product Name

Human Interferon-induced protein with tetratricopeptide repeats 1, IFIT1 ELISA Kit

Product Gene Name

IFIT1 elisa kit

Product Synonym Names

Human Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) ELISA kit; G10P1; GARG-16; IFI-56; IFI56; IFNAI1; ISG56; RNM561; Interferon; alpha-inducible protein (MW 56kD) ; interferon-induced protein 56; interferon-induced protein with tetratricopeptide repeats 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

< 3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human IFIT1. No significant cross-reactivity or interference between human IFIT1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

55,360 Da

OMIM

147690

3D Structure

ModBase 3D Structure for P09914

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IFIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFIT1 purchase

MBS925861 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFIT1. The ELISA analytical biochemical technique of the MBS925861 kit is based on IFIT1 antibody-IFIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IFIT1. It may not necessarily be applicable to this product.

NCBI GI #

400286381

NCBI GeneID

3434

NCBI Accession #

NP_001257857.1

UniProt Primary Accession #

P09914

UniProt Secondary Accession #

Q5T7J1; Q96QM5; B3KS50; D3DR31

UniProt Related Accession #

P09914

GenBank Nucleotide #

NM_001270928.1

NCBI Official Full Name

interferon-induced protein with tetratricopeptide repeats 1 isoform 3

NCBI Official Synonym Full Names

interferon-induced protein with tetratricopeptide repeats 1

NCBI Official Symbol

IFIT1

NCBI Official Synonym Symbols

C56; P56; G10P1; IFI56; ISG56; IFI-56; IFIT-1; IFNAI1; RNM561; IFI-56K

UniProt Protein Name

Interferon-induced protein with tetratricopeptide repeats 1

UniProt Synonym Protein Names

Interferon-induced 56 kDa protein; IFI-56K; P56

Protein Family

Interferon-induced protein with tetratricopeptide repeats

UniProt Gene Name

IFIT1

UniProt Synonym Gene Names

G10P1; IFI56; IFNAI1; ISG56; IFIT-1; IFI-56K; P56

UniProt Entry Name

IFIT1_HUMAN

NCBI Protein Information

interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561; interferon-induced 56 kDa protein; interferon, alpha-inducible protein (MW 56kD)

NCBI Summary for IFIT1

This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012]

UniProt Comments for IFIT1

IFIT1: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O- methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses. Belongs to the IFIT family.

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; RNA binding

Biological Process: intracellular transport of viral proteins in host cell; positive regulation of viral genome replication; negative regulation of helicase activity; negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; negative regulation of defense response to virus by host; negative regulation of protein binding; defense response to virus

Research Articles on IFIT1

1. The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IFIT1 elisa kit

Reference Product	PubMed Publications
IFIT3 elisa kit	>13 publications with IFIT1 and IFIT3
EIF3E elisa kit	>4 publications with IFIT1 and EIF3E

Pathways associated with IFIT1 elisa kit

Products by Pathway	Pathway Diagram
Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies	Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
ISG15 Antiviral Mechanism Pathway antibodies	ISG15 Antiviral Mechanism Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interferon Signaling Pathway antibodies	Interferon Signaling Pathway Diagram
Interferon Alpha/beta Signaling Pathway antibodies	Interferon Alpha/beta Signaling Pathway Diagram

Diseases associated with IFIT1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with IFIT1 and Neoplasms
Nervous System Diseases Antibodies	>11 publications with IFIT1 and Nervous System Diseases
Inflammation Antibodies	>8 publications with IFIT1 and Inflammation
Skin Diseases Antibodies	>6 publications with IFIT1 and Skin Diseases
Liver Diseases Antibodies	>5 publications with IFIT1 and Liver Diseases
Necrosis Antibodies	>4 publications with IFIT1 and Necrosis
Lymphoma Antibodies	>3 publications with IFIT1 and Lymphoma
Disease Models, Animal Antibodies	>2 publications with IFIT1 and Disease Models, Animal
Melanoma Antibodies	>2 publications with IFIT1 and Melanoma
Fever Antibodies	>2 publications with IFIT1 and Fever

Organs/Tissues associated with IFIT1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>27 publications with IFIT1 and Blood
Liver Antibodies	>6 publications with IFIT1 and Liver
Brain Antibodies	>6 publications with IFIT1 and Brain
Lung Antibodies	>5 publications with IFIT1 and Lung
Bone Antibodies	>4 publications with IFIT1 and Bone
Kidney Antibodies	>3 publications with IFIT1 and Kidney
Muscle Antibodies	>2 publications with IFIT1 and Muscle
Uterus Antibodies	>2 publications with IFIT1 and Uterus
Skin Antibodies	>1 publications with IFIT1 and Skin
Uncharacterized Tissue Antibodies	>1 publications with IFIT1 and Uncharacterized Tissue