CD247 Molecule (CD247) ELISA Kit for Human

Name: Human CD247 ELISA Kit; CD247 Molecule; MyBioSource
Brand: MyBioSource
SKU: MBS926331
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS926331

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD247 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD247 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

CD247 molecule, ELISA Kit

Full Product Name

Human T-cell surface glycoprotein CD3 zeta chain, CD247 ELISA Kit

Product Gene Name

CD247 elisa kit

Product Synonym Names

Human T-cell surface glycoprotein CD3 zeta chain (CD247) ELISA kit; CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ; CD247 antigen; zeta subunit; CD3Z antigen; zeta polypeptide (TiT3 complex) ; T-cell antigen receptor complex; zeta subunit of CD3; T-cell receptor T3 zeta chain; T-; CD247 molecule

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

0.156 ng/ml -10 ng/ml.

Sensitivity

The minimum detectable dose of human CD247 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human CD247. No significant cross-reactivity or interference between human CD247 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

18,696 Da

OMIM

gene 610163

3D Structure

ModBase 3D Structure for P20963

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CD247 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD247 purchase

MBS926331 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD247 molecule, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD247. The ELISA analytical biochemical technique of the MBS926331 kit is based on CD247 antibody-CD247 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD247 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD247. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CD247. It may not necessarily be applicable to this product.

NCBI GI #

4557431

NCBI GeneID

919

NCBI Accession #

NP_000725.1

UniProt Primary Accession #

P20963

UniProt Secondary Accession #

Q5VX13; Q8TAX4; B1AK49

UniProt Related Accession #

P20963; A46461

GenBank Nucleotide #

NM_000734.3

NCBI Official Full Name

T-cell surface glycoprotein CD3 zeta chain isoform 2

NCBI Official Synonym Full Names

CD247 molecule

NCBI Official Symbol

CD247

NCBI Official Synonym Symbols

T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA

UniProt Protein Name

T-cell surface glycoprotein CD3 zeta chain

UniProt Synonym Protein Names

T-cell receptor T3 zeta chain; CD_antigen: CD247

Protein Family

T-cell surface glycoprotein

UniProt Gene Name

CD247

UniProt Synonym Gene Names

CD3Z; T3Z; TCRZ

UniProt Entry Name

CD3Z_HUMAN

NCBI Protein Information

T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3

NCBI Summary for CD247

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for CD247

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25

Research Articles on CD247

1. Two transcription factor binding sites and a long non-coding RNA are identified within the Cd247 gene.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD247 elisa kit

Reference Product	PubMed Publications
ZAP70 elisa kit	>2 publications with CD247 and ZAP70
SHC1 elisa kit	>1 publications with CD247 and SHC1
SH2B3 elisa kit	>1 publications with CD247 and SH2B3
PTPRC elisa kit	>1 publications with CD247 and PTPRC
FYN elisa kit	>1 publications with CD247 and FYN
CD3D elisa kit	>1 publications with CD247 and CD3D

Pathways associated with CD247 elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
CXCR4-mediated Signaling Events Pathway antibodies	CXCR4-mediated Signaling Events Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream TCR Signaling Pathway antibodies	Downstream TCR Signaling Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Generation Of Second Messenger Molecules Pathway antibodies	Generation Of Second Messenger Molecules Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram

Diseases associated with CD247 elisa kit

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>5 publications with CD247 and Skin Diseases
Autoimmune Diseases Antibodies	>4 publications with CD247 and Autoimmune Diseases
Scleroderma, Diffuse Antibodies	>4 publications with CD247 and Scleroderma, Diffuse
Scleroderma, Systemic Antibodies	>4 publications with CD247 and Scleroderma, Systemic
Neoplasms Antibodies	>3 publications with CD247 and Neoplasms
Fibrosis Antibodies	>2 publications with CD247 and Fibrosis
Inflammation Antibodies	>2 publications with CD247 and Inflammation
Vascular Diseases Antibodies	>1 publications with CD247 and Vascular Diseases
Breast Neoplasms Antibodies	>1 publications with CD247 and Breast Neoplasms
Congenital Abnormalities Antibodies	>1 publications with CD247 and Congenital Abnormalities

Organs/Tissues associated with CD247 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>9 publications with CD247 and Blood