Centromere Protein E (CENPE) ELISA Kit for Canine

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq, Jul 2008]

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: microtubule cytoskeleton; kinetochore; microtubule; kinesin complex; membrane; cytoplasm; outer kinetochore of condensed chromosome; midbody; chromosome; nucleus; cytosol; chromosome, pericentric region

Molecular Function: kinetochore binding; protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: kinetochore assembly; attachment of spindle microtubules to kinetochore during mitosis; cell division; metabolic process; positive regulation of protein kinase activity; multicellular organismal development; regulation of mitotic metaphase/anaphase transition; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; blood coagulation; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; microtubule-based movement; chromosome segregation

Disease: Microcephaly 13, Primary, Autosomal Recessive