Tyrosine Hydroxylase (TH) ELISA Kit for Mouse

Name: Mouse TH ELISA Kit; Tyrosine Hydroxylase; MyBioSource
Brand: MyBioSource
SKU: MBS926829
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS926829

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

tyrosine hydroxylase (TH), ELISA Kit

Full Product Name

Mouse tyrosine hydroxylase, TH ELISA Kit

Product Gene Name

TH elisa kit

Product Synonym Names

Mouse tyrosine hydroxylase (TH) ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; tyrosine hydroxylase (TH)

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell culture supernates

Detection Range

62.5 pg/ml-4000 pg/ml

Sensitivity

< 15.6 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse TH. No significant cross-reactivity or interference between mouse TH and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

55,993 Da

3D Structure

ModBase 3D Structure for P24529

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TH purchase

MBS926829 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tyrosine hydroxylase (TH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TH. The ELISA analytical biochemical technique of the MBS926829 kit is based on TH antibody-TH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.

NCBI GI #

6678337

NCBI GeneID

21823

NCBI Accession #

NP_033403.1

UniProt Primary Accession #

P24529

UniProt Related Accession #

P24529

GenBank Nucleotide #

NM_009377.1

NCBI Official Full Name

tyrosine 3-monooxygenase

NCBI Official Synonym Full Names

tyrosine hydroxylase

NCBI Official Symbol

UniProt Protein Name

Tyrosine 3-monooxygenase

UniProt Synonym Protein Names

Tyrosine 3-hydroxylase; TH

UniProt Gene Name

UniProt Synonym Gene Names

UniProt Entry Name

TY3H_MOUSE

NCBI Protein Information

tyrosine 3-monooxygenase; tyrosine 3-hydroxylase

UniProt Comments for TH

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Vesicle; EC 1.14.16.2; Oxidoreductase; Mitochondrial

Cellular Component: internal side of plasma membrane; neuron projection; cytoplasmic vesicle membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cell soma; axon; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: protein domain specific binding; ferric iron binding; metal ion binding; ferrous iron binding; oxidoreductase activity; oxygen binding; tyrosine 3-monooxygenase activity; amino acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; dopamine binding; monooxygenase activity

Biological Process: synaptic transmission, dopaminergic; eating behavior; heart development; mating behavior; locomotory behavior; learning; dopamine biosynthetic process; memory; synaptic vesicle amine transport; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; organ morphogenesis; eye photoreceptor cell development; response to ethanol; catecholamine biosynthetic process; visual perception; aromatic amino acid family metabolic process; response to hypoxia; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; neurotransmitter biosynthetic process

Research Articles on TH

1. Mating increases neuronal tyrosine hydroxylase expression and selectively gates transmission of male chemosensory information in female mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TH elisa kit

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amine-derived Hormones Pathway antibodies	Amine-derived Hormones Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Catecholamine Biosynthesis Pathway antibodies	Catecholamine Biosynthesis Pathway Diagram
Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies	Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies	Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram

Diseases associated with TH elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1099131 publications with TH and Neoplasms
Cardiovascular Diseases Antibodies	>878870 publications with TH and Cardiovascular Diseases
Nervous System Diseases Antibodies	>806657 publications with TH and Nervous System Diseases
Heart Diseases Antibodies	>445050 publications with TH and Heart Diseases
Brain Diseases Antibodies	>381290 publications with TH and Brain Diseases
Mental Disorders Antibodies	>373276 publications with TH and Mental Disorders
Gastrointestinal Diseases Antibodies	>355845 publications with TH and Gastrointestinal Diseases
Pain Antibodies	>288165 publications with TH and Pain
Kidney Diseases Antibodies	>193724 publications with TH and Kidney Diseases
Myocardial Ischemia Antibodies	>189746 publications with TH and Myocardial Ischemia

Organs/Tissues associated with TH elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>250686 publications with TH and Brain
Ovary Antibodies	>20821 publications with TH and Ovary
Embryonic Tissue Antibodies	>15110 publications with TH and Embryonic Tissue
Sympathetic Ganglion Antibodies	>2366 publications with TH and Sympathetic Ganglion