Cytidine Deaminase (CDA) ELISA Kit for Human

Name: Human CDA ELISA Kit; Cytidine Deaminase; MyBioSource
Brand: MyBioSource
SKU: MBS928959
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS928959

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDA present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

cytidine deaminase (CDA), ELISA Kit

Full Product Name

Human Cytidine deaminase, CDA ELISA Kit

Product Gene Name

CDA elisa kit

Product Synonym Names

Human Cytidine deaminase (CDA) ELISA kit; CDD; cytidine aminohydrolase; cytosine nucleoside deaminase; small cytidine deaminase; cytidine deaminase

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

15.6 pg/ml-1000 pg/ml

Sensitivity

3.9 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Human CDA. No significant cross-reactivity or interference between Human CDA and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

16,185 Da

OMIM

123920

3D Structure

ModBase 3D Structure for P32320

Detection Wavelength

450 nm

Sample Volume

50-100ul

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CDA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDA purchase

MBS928959 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytidine deaminase (CDA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDA. The ELISA analytical biochemical technique of the MBS928959 kit is based on CDA antibody-CDA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CDA. It may not necessarily be applicable to this product.

NCBI GI #

11386157

NCBI GeneID

978

NCBI Accession #

NP_001776.1

UniProt Primary Accession #

P32320

UniProt Related Accession #

P32320

GenBank Nucleotide #

NM_001785.2

NCBI Official Full Name

cytidine deaminase

NCBI Official Synonym Full Names

cytidine deaminase

NCBI Official Symbol

CDA

NCBI Official Synonym Symbols

CDD

UniProt Protein Name

Cytidine deaminase

UniProt Synonym Protein Names

Cytidine aminohydrolase

Protein Family

Calcium-transporting ATPase

UniProt Gene Name

CDA

UniProt Synonym Gene Names

CDD

UniProt Entry Name

CDD_HUMAN

NCBI Protein Information

cytidine deaminase; cytidine aminohydrolase; small cytidine deaminase; cytosine nucleoside deaminase

NCBI Summary for CDA

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]

UniProt Comments for CDA

CDA: This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; EC 3.5.4.5

Chromosomal Location of Human Ortholog: 1p36.2-p35

Cellular Component: extracellular region; cytosol

Molecular Function: protein homodimerization activity; zinc ion binding; nucleoside binding; cytidine deaminase activity

Biological Process: cell surface receptor linked signal transduction; pyrimidine base metabolic process; cytosine metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; cytidine deamination; negative regulation of cell growth; pyrimidine salvage; negative regulation of nucleotide metabolic process; protein homotetramerization

Research Articles on CDA

1. The T70 variant has a lower catalytic efficiency toward the analyzed substrates when compared to the A70 variant, suggesting that patients carrying the 208G>A SNP may have a greater exposure to cytosine based pro drugs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CDA elisa kit

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Salvage Reactions Pathway antibodies	Pyrimidine Salvage Reactions Pathway Diagram
Salvage Pathways Of Pyrimidine Deoxyribonucleotides antibodies	Salvage Pathways Of Pyrimidine Deoxyribonucleotides Diagram

Diseases associated with CDA elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>551 publications with CDA and Neoplasms
Hematologic Diseases Antibodies	>327 publications with CDA and Hematologic Diseases
Anemia Antibodies	>238 publications with CDA and Anemia
Drug Toxicity Antibodies	>208 publications with CDA and Drug Toxicity
Anemia, Hemolytic Antibodies	>191 publications with CDA and Anemia, Hemolytic
Lymphoma Antibodies	>126 publications with CDA and Lymphoma
Bone Marrow Diseases Antibodies	>82 publications with CDA and Bone Marrow Diseases
Neutropenia Antibodies	>80 publications with CDA and Neutropenia
Thrombocytopenia Antibodies	>69 publications with CDA and Thrombocytopenia
Lymphoma, Non-Hodgkin Antibodies	>68 publications with CDA and Lymphoma, Non-Hodgkin

Organs/Tissues associated with CDA elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>461 publications with CDA and Blood
Bone Antibodies	>258 publications with CDA and Bone
Bone Marrow Antibodies	>219 publications with CDA and Bone Marrow
Liver Antibodies	>55 publications with CDA and Liver
Brain Antibodies	>53 publications with CDA and Brain
Kidney Antibodies	>50 publications with CDA and Kidney
Skin Antibodies	>47 publications with CDA and Skin
Lung Antibodies	>33 publications with CDA and Lung
Spleen Antibodies	>30 publications with CDA and Spleen
Eye Antibodies	>15 publications with CDA and Eye