Prolylcarboxypeptidase (Angiotensinase C) (PRCP) ELISA Kit for Mouse

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRCP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of PRCP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS929190 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prolylcarboxypeptidase (angiotensinase C) (PRCP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRCP. The ELISA analytical biochemical technique of the MBS929190 kit is based on PRCP antibody-PRCP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRCP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRCP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA Kit is quantitative determination of mouse lysosomal prolylcarboxypeptidase or PRCP.

Target protein, PRCP, is peptidase that catalyzes hydrolysis of amide bond between proline and C-terminal amino acid; its functions include cleavage of proline-linked amino acids on C-terminus of peptide hormones such as angiotensin III and des-Arg(9)-bradykinin.

PRCP is also known as lysosomal Pro-X carboxypeptidase, proline carboxypeptidase and angiotensinase C in NCBI and UniProt data bases.

Test system is designed as sandwich-type ELISA and concentration of PRCP is measured using pair of antibodies specific to target protein.

Kit is applicable for testing variable types of samples, including serum, plasma, tissue homogenates and cell lysates.

Product contains microwell plate pre-coated with capture antibodies against PRCP and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of PRCP using biotinylated detector antibodies against PRCP, avidin-HRP conjugate, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 9.37pg/mL and its reportable range is 37.5-2400pg/mL; concentrated calibrator’s preparation for standard curve is provided in lyophilized form and requires dilution for making working levels of calibrators.

Intra- and inter-assay CV values, reported as kit’s precision characteristics, are not greater than 8% and 10%, respectively.

Assay doesn’t show significant cross-reactivity with analogous proteins.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8°C until indicated expiration date.