Lipase, Hormone-Sensitive (LIPE) ELISA Kit for Mouse

Name: Mouse LIPE ELISA Kit; Lipase, Hormone-Sensitive; MyBioSource
Brand: MyBioSource
SKU: MBS929695
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS929695

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LIPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIPE present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

lipase, hormone-sensitive (LIPE), ELISA Kit

Full Product Name

Mouse Hormone-sensitive lipase, LIPE ELISA Kit

Product Gene Name

LIPE elisa kit

Product Synonym Names

Mouse Hormone-sensitive lipase (LIPE) ELISA kit; HSL; LHS; hormone-sensitive lipase testicular isoform; lipase E; lipase; hormone-sensitive

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.156 U/ml-10 U/ml

Sensitivity

0.039 U/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse LIPE. No significant cross-reactivity or interference between Mouse LIPE and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.

Molecular Weight

83,348 Da

3D Structure

ModBase 3D Structure for P54310

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Biosynthesis/Metabolism

Protein Biological Process 2

Lipogenesis and lipometabolism

Protein Biological Process 3

Cholesterol metabolism

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LIPE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIPE purchase

MBS929695 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lipase, hormone-sensitive (LIPE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LIPE. The ELISA analytical biochemical technique of the MBS929695 kit is based on LIPE antibody-LIPE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LIPE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LIPE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LIPE. It may not necessarily be applicable to this product.

NCBI GI #

87239972

NCBI GeneID

16890

NCBI Accession #

NP_001034596.1

UniProt Primary Accession #

P54310

UniProt Secondary Accession #

P97866; Q3TE34; Q6GU16; Q8CDI9

UniProt Related Accession #

P54310

GenBank Nucleotide #

NM_001039507.2

NCBI Official Full Name

hormone-sensitive lipase isoform 2

NCBI Official Synonym Full Names

lipase, hormone sensitive

NCBI Official Symbol

Lipe

NCBI Official Synonym Symbols

HSL; 4933403G17Rik

UniProt Protein Name

Hormone-sensitive lipase

Protein Family

Hormone-sensitive lipase

UniProt Gene Name

Lipe

UniProt Synonym Gene Names

HSL

UniProt Entry Name

LIPS_MOUSE

NCBI Protein Information

hormone-sensitive lipase

UniProt Comments for LIPE

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: EC 3.1.1.79; Lipase

Cellular Component: extracellular space; mitochondrion; membrane; cytoplasm; plasma membrane; lipid particle; caveola; cytosol; nucleus

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; rRNA primary transcript binding; hydrolase activity; lipase activity; acylglycerol lipase activity; hydrolase activity, acting on ester bonds; protein kinase binding

Biological Process: steroid metabolic process; cholesterol metabolic process; metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; lipid metabolic process; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; lipid catabolic process; long-chain fatty acid catabolic process

Research Articles on LIPE

1. Changes in fatty acid metabolism observed in testes from HSL-knockout male mice may underlie the infertility observed in these animals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIPE elisa kit

Reference Product	PubMed Publications
Prkaca elisa kit	>1 publications with LIPE and Prkaca

Pathways associated with LIPE elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram
Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway antibodies	Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Triacylglyceride Synthesis Pathway antibodies	Triacylglyceride Synthesis Pathway Diagram

Diseases associated with LIPE elisa kit

Disease Name	Pubmed Publications
Cognition Disorders Antibodies	>17 publications with LIPE and Cognition Disorders
Obesity Antibodies	>16 publications with LIPE and Obesity
Cardiovascular Diseases Antibodies	>10 publications with LIPE and Cardiovascular Diseases
Insulin Resistance Antibodies	>10 publications with LIPE and Insulin Resistance
Vascular Diseases Antibodies	>9 publications with LIPE and Vascular Diseases
Drug Toxicity Antibodies	>8 publications with LIPE and Drug Toxicity
Neoplasms Antibodies	>6 publications with LIPE and Neoplasms
Atherosclerosis Antibodies	>6 publications with LIPE and Atherosclerosis
Muscular Diseases Antibodies	>6 publications with LIPE and Muscular Diseases
Hypertension Antibodies	>4 publications with LIPE and Hypertension

Organs/Tissues associated with LIPE elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>28 publications with LIPE and Brain
Connective Tissue Antibodies	>12 publications with LIPE and Connective Tissue
Kidney Antibodies	>5 publications with LIPE and Kidney
Embryonic Tissue Antibodies	>4 publications with LIPE and Embryonic Tissue
Lung Antibodies	>3 publications with LIPE and Lung
Testis Antibodies	>3 publications with LIPE and Testis
Eye Antibodies	>3 publications with LIPE and Eye
Skin Antibodies	>2 publications with LIPE and Skin
Intestine Antibodies	>2 publications with LIPE and Intestine
Prostate Antibodies	>2 publications with LIPE and Prostate