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Agrin (AGRN) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS930009

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGRN present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
agrin (AGRN), ELISA Kit
Full Product Name
Mouse Agrin, AGRN ELISA Kit
Product Gene Name
AGRN elisa kit
Product Synonym Names
Mouse Agrin (AGRN) ELISA kit; RP11-54O7.13; FLJ45064; agrin proteoglycan; agrin
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse AGRN. No significant cross-reactivity or interference between Mouse AGRN and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Molecular Weight
207,539 Da
3D Structure
ModBase 3D Structure for A2ASQ1
Detection Wavelength
450 nm
Sample Volume
50-100ul
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of AGRN elisa kit

Mouse AGRN ELISA Kit – Cat# MBS930009

Other Notes

Small volumes of AGRN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AGRN purchase

MBS930009 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the agrin (AGRN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGRN. The ELISA analytical biochemical technique of the MBS930009 kit is based on AGRN antibody-AGRN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGRN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGRN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AGRN. It may not necessarily be applicable to this product.

NCBI GI #
218563482
NCBI GeneID
11603
NCBI Accession #
A2ASQ1.1
UniProt Primary Accession #
A2ASQ1
UniProt Secondary Accession #
A2ASP9; A2ASQ0; B2RWU1
UniProt Related Accession #
A2ASQ1
NCBI Official Full Name
Agrin
NCBI Official Synonym Full Names
agrin
NCBI Official Symbol
Agrn
NCBI Official Synonym Symbols
Agrin; nmf380
UniProt Protein Name
Agrin
Protein Family
Agrin
UniProt Gene Name
Agrn
UniProt Synonym Gene Names
Agrin; C90; C22
UniProt Entry Name
AGRIN_MOUSE
NCBI Protein Information
agrin

UniProt Comments for AGRN

AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.

Protein type: Extracellular matrix; Cell development/differentiation; Secreted, signal peptide; Cell adhesion; Secreted

Cellular Component: extracellular matrix; extracellular space; cell surface; membrane; cytoplasm; plasma membrane; integral to membrane; basement membrane; basal lamina; synapse; cell junction; sarcolemma

Molecular Function: heparan sulfate proteoglycan binding; protein binding; transforming growth factor beta binding; ATPase inhibitor activity; acetylcholine receptor regulator activity; calcium ion binding

Biological Process: positive regulation of filopodium formation; regulation of GTPase activity; regulation of protein amino acid phosphorylation; positive regulation of protein binding; neurotransmitter receptor metabolic process; multicellular organismal development; positive regulation of synaptic growth at neuromuscular junction; receptor clustering; synaptic transmission; synaptogenesis; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; protein heterotetramerization; positive regulation of transcription from RNA polymerase II promoter; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; regulation of receptor activity; cell differentiation; neuromuscular junction development
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Research Articles on AGRN

1. reviews the evidence that agrin regulates synapse development, plasticity and signaling in the brain and discusses the evidence for the proposed mechanisms

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AGRN elisa kit

 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with AGRN and FLNA

Pathways associated with AGRN elisa kit

 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram

Organs/Tissues associated with AGRN elisa kit

 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >5 publications with AGRN and Other
 Embryonic Tissue Antibodies  >4 publications with AGRN and Embryonic Tissue
 Brain Antibodies  >4 publications with AGRN and Brain
 Blood Antibodies  >3 publications with AGRN and Blood
 Heart Antibodies  >1 publications with AGRN and Heart
 Bone Antibodies  >1 publications with AGRN and Bone
 Dorsal Root Ganglion Antibodies  >1 publications with AGRN and Dorsal Root Ganglion
 Kidney Antibodies  >1 publications with AGRN and Kidney
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