Ectodysplasin A (EDA) ELISA Kit for Mouse

Name: Mouse EDA ELISA Kit; Ectodysplasin A; MyBioSource
Brand: MyBioSource
SKU: MBS930835
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS930835

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDA present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

ectodysplasin A (EDA), ELISA Kit

Full Product Name

Mouse Ectodysplasin-A, EDA ELISA Kit

Product Gene Name

EDA elisa kit

Product Synonym Names

Mouse Ectodysplasin-A (EDA) ELISA kit; RP11-351K23.1; ED1; ED1-A1; ED1-A2; EDA1; EDA2; HED; XHED; XLHED; X-linked anhidroitic ectodermal dysplasia protein; ectodysplasin A

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

15.6 pg/ml -1000 pg/ml

Sensitivity

Typically less than 3.9 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse EDA. No significant cross-reactivity or interference between mouse EDA and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

41,603 Da

3D Structure

ModBase 3D Structure for O54693

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of EDA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EDA purchase

MBS930835 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ectodysplasin A (EDA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EDA. The ELISA analytical biochemical technique of the MBS930835 kit is based on EDA antibody-EDA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EDA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EDA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EDA. It may not necessarily be applicable to this product.

NCBI GI #

295789018

NCBI GeneID

13607

NCBI Accession #

NP_001171408.1

UniProt Primary Accession #

O54693

UniProt Secondary Accession #

O35705; Q9QWJ8; Q9QZ01; Q9QZ02; B7ZMT7

UniProt Related Accession #

O54693

GenBank Nucleotide #

NM_001177937.1

NCBI Official Full Name

ectodysplasin-A isoform 2

NCBI Official Synonym Full Names

ectodysplasin-A

NCBI Official Symbol

Eda

NCBI Official Synonym Symbols

Ta; Ed1; HED; EDA1; XLHED; tabby; Eda-A1; Eda-A2

UniProt Protein Name

Ectodysplasin-A

UniProt Synonym Protein Names

EDA protein homolog; Tabby proteinCleaved into the following 2 chains:Ectodysplasin-A, membrane form; Ectodysplasin-A, secreted form

Protein Family

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

UniProt Gene Name

Eda

UniProt Synonym Gene Names

Ed1; Ta

UniProt Entry Name

EDA_MOUSE

NCBI Protein Information

ectodysplasin-A; EDA protein homolog

UniProt Comments for EDA

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Cellular Component: endoplasmic reticulum membrane; collagen; membrane; integral to plasma membrane; apical part of cell; integral to membrane; plasma membrane; extracellular region

Molecular Function: tumor necrosis factor receptor binding

Biological Process: skin development; positive regulation of I-kappaB kinase/NF-kappaB cascade; pigmentation; hair follicle development; cell-matrix adhesion; multicellular organismal development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; cell differentiation; odontogenesis of dentine-containing teeth

Research Articles on EDA

1. Eda and activin A are upstream regulators of Foxi3 in skin appendage placodes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EDA elisa kit

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with EDA elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>148 publications with EDA and Nervous System Diseases
Necrosis Antibodies	>142 publications with EDA and Necrosis
Brain Diseases Antibodies	>80 publications with EDA and Brain Diseases
Heart Diseases Antibodies	>70 publications with EDA and Heart Diseases
Inflammation Antibodies	>67 publications with EDA and Inflammation
Ectodermal Dysplasia 1, Anhidrotic Antibodies	>50 publications with EDA and Ectodermal Dysplasia 1, Anhidrotic
Adenocarcinoma Antibodies	>46 publications with EDA and Adenocarcinoma
Liver Diseases Antibodies	>35 publications with EDA and Liver Diseases
Kidney Diseases Antibodies	>27 publications with EDA and Kidney Diseases
Depressive Disorder Antibodies	>25 publications with EDA and Depressive Disorder

Organs/Tissues associated with EDA elisa kit

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>355 publications with EDA and Other
Skin Antibodies	>280 publications with EDA and Skin
Brain Antibodies	>144 publications with EDA and Brain
Lung Antibodies	>70 publications with EDA and Lung
Liver Antibodies	>57 publications with EDA and Liver
Embryonic Tissue Antibodies	>45 publications with EDA and Embryonic Tissue
Joint Antibodies	>21 publications with EDA and Joint
Salivary Gland Antibodies	>12 publications with EDA and Salivary Gland
Mammary Gland Antibodies	>8 publications with EDA and Mammary Gland
Bladder Antibodies	>4 publications with EDA and Bladder