Glutaminyl-Peptide Cyclotransferase (QPCT) ELISA Kit for Human

Name: Human QPCT ELISA Kit; Glutaminyl-Peptide Cyclotransferase; MyBioSource
Brand: MyBioSource
SKU: MBS931378
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS931378

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for QPCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QPCT present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

glutaminyl-peptide cyclotransferase (QPCT), ELISA Kit

Full Product Name

Human Glutaminyl-peptide cyclotransferase, QPCT ELISA Kit

Product Gene Name

QPCT elisa kit

Product Synonym Names

Human Glutaminyl-peptide cyclotransferase (QPCT) ELISA kit; GCT; QC; glutaminyl cyclase; glutaminyl-peptide cyclotransferase

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates.

Detection Range

0.312 ng/ml -20 ng/ml.

Sensitivity

0.078 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human QPCT. No significant cross-reactivity or interference between human QPCT and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

40,877 Da

OMIM

607065

3D Structure

ModBase 3D Structure for Q16769

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of QPCT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for QPCT purchase

MBS931378 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutaminyl-peptide cyclotransferase (QPCT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing QPCT. The ELISA analytical biochemical technique of the MBS931378 kit is based on QPCT antibody-QPCT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect QPCT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, QPCT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for QPCT. It may not necessarily be applicable to this product.

NCBI GI #

6912618

NCBI GeneID

25797

NCBI Accession #

NP_036545.1

UniProt Primary Accession #

Q16769

UniProt Secondary Accession #

Q16770; Q3KRG6; Q53TR4

UniProt Related Accession #

Q16769

GenBank Nucleotide #

NM_012413.3

NCBI Official Full Name

glutaminyl-peptide cyclotransferase

NCBI Official Synonym Full Names

glutaminyl-peptide cyclotransferase

NCBI Official Symbol

QPCT

NCBI Official Synonym Symbols

QC; GCT; sQC

UniProt Protein Name

Glutaminyl-peptide cyclotransferase

UniProt Synonym Protein Names

Glutaminyl cyclase; QC; sQC; Glutaminyl-tRNA cyclotransferase; Glutamyl cyclase; EC

Protein Family

Glutaminyl-peptide cyclotransferase

UniProt Gene Name

QPCT

UniProt Synonym Gene Names

QC; sQC; EC

UniProt Entry Name

QPCT_HUMAN

NCBI Protein Information

glutaminyl-peptide cyclotransferase; EC; glutamyl cyclase; glutaminyl cyclase; glutaminyl-tRNA cyclotransferase

NCBI Summary for QPCT

This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. [provided by RefSeq, Jul 2008]

UniProt Comments for QPCT

QPCT: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. Belongs to the glutaminyl-peptide cyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Transferase; EC 2.3.2.5; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p22.2

Molecular Function: glutaminyl-peptide cyclotransferase activity; zinc ion binding

Biological Process: peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase; protein modification process

Research Articles on QPCT

1. this study demonistrated that QPCT mRNAand protein from mononuclear cells correlated with theseverity of dementia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with QPCT elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with QPCT and Inflammation
Brain Diseases Antibodies	>1 publications with QPCT and Brain Diseases
Nervous System Diseases Antibodies	>1 publications with QPCT and Nervous System Diseases
Fibrosis Antibodies	>1 publications with QPCT and Fibrosis
Kidney Diseases Antibodies	>1 publications with QPCT and Kidney Diseases
Neoplasms, Experimental Antibodies	>1 publications with QPCT and Neoplasms, Experimental
Hyperplasia Antibodies	>1 publications with QPCT and Hyperplasia
Disease Models, Animal Antibodies	>1 publications with QPCT and Disease Models, Animal
Hypertension Antibodies	>1 publications with QPCT and Hypertension
Leukemia, Lymphocytic, Chronic, B-Cell Antibodies	>1 publications with QPCT and Leukemia, Lymphocytic, Chronic, B-Cell

Organs/Tissues associated with QPCT elisa kit

Organ/Tissue Name	Pubmed Publications
Thyroid Antibodies	>3 publications with QPCT and Thyroid
Bone Antibodies	>3 publications with QPCT and Bone
Brain Antibodies	>3 publications with QPCT and Brain
Pituitary Gland Antibodies	>2 publications with QPCT and Pituitary Gland
Blood Antibodies	>2 publications with QPCT and Blood
Eye Antibodies	>1 publications with QPCT and Eye
Kidney Antibodies	>1 publications with QPCT and Kidney
Connective Tissue Antibodies	>1 publications with QPCT and Connective Tissue