Chemokine (C-X-C Motif) Ligand 3 (CXCL3) ELISA Kit for Mouse

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of CXCL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS932211 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chemokine (C-X-C motif) ligand 3 (CXCL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CXCL3. The ELISA analytical biochemical technique of the MBS932211 kit is based on CXCL3 antibody-CXCL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). CXCL3, aka GRO gamma, is a small cytokine with migratory promoting functions and critical for brain development. A member of the chemokine family based on chemokine motif ligand 3 homology. Loss of CXCL3 interactions with CCR ( C X C chemokine motif receptor) has been suggest to switch neurons to a proliferative phenotype, thus generating interest in CXCL3 GRO gamma as a therapeutic target in brain tumors. Quantitation of mouse but not rat CXCL3 or human protein is possible with sandwich format ELISA kit. This CXCL3 ELISA kit chemokine has been extensively validated, has excellent sensitivity and pg ml detection. Included in these ELISA kits are all products required to perform CXCL 3 assay on your samples, a 96 tests plate, standard CCL protein and all antibodies. Protocol details are found for CXCL ligand 3 protein ELISA kit 1 included datasheet.