Collagen, Type V, Alpha 1 (COL5A1) ELISA Kit for Mouse

Name: Mouse COL5A1 ELISA Kit; Collagen, Type V, Alpha 1; MyBioSource
Brand: MyBioSource
SKU: MBS932248
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS932248

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL5A1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

collagen, type V, alpha 1 (COL5A1), ELISA Kit

Full Product Name

Mouse Collagen alpha-1 (V) chain, COL5A1 ELISA Kit

Product Gene Name

COL5A1 elisa kit

Product Synonym Names

Mouse Collagen alpha-1 (V) chain (COL5A1) ELISA kit; RP11-263F14.1; alpha 1 type V collagen; collagen; type V; alpha 1

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Serum, Plasma And Tissue Homogenates

Detection Range

0.312 ng/ml-20 ng/ml

Sensitivity

0.078 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human COL5A1. No significant cross-reactivity or interference between human COL5A1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

183,677 Da

3D Structure

ModBase 3D Structure for O88207

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of COL5A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL5A1 purchase

MBS932248 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the collagen, type V, alpha 1 (COL5A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL5A1. The ELISA analytical biochemical technique of the MBS932248 kit is based on COL5A1 antibody-COL5A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL5A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL5A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for COL5A1. It may not necessarily be applicable to this product.

NCBI GI #

164565411

NCBI GeneID

12831

NCBI Accession #

NP_056549.2

UniProt Primary Accession #

O88207

UniProt Secondary Accession #

Q3UG94; Q6F6B0; A3KGE3

UniProt Related Accession #

O88207

GenBank Nucleotide #

NM_015734.2

NCBI Official Full Name

collagen alpha-1(V) chain

NCBI Official Synonym Full Names

collagen, type V, alpha 1

NCBI Official Symbol

Col5a1

NCBI Official Synonym Symbols

AI413331

UniProt Protein Name

Collagen alpha-1(V) chain

Protein Family

Collagen

UniProt Gene Name

Col5a1

UniProt Entry Name

CO5A1_MOUSE

NCBI Protein Information

collagen alpha-1(V) chain; collagen alpha-1(V) chain; alpha1(V) collagen; pro-alpha1(V) collagen; procollagen type V alpha 1; procollagen, type V, alpha 1

UniProt Comments for COL5A1

COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family.

Protein type: Secreted; Extracellular matrix; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; collagen type V; extracellular region; basement membrane

Molecular Function: heparin binding; protein binding; proteoglycan binding; extracellular matrix structural constituent; platelet-derived growth factor binding

Biological Process: heart morphogenesis; skin development; blood vessel development; cell migration; integrin biosynthetic process; collagen fibril organization; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells

Research Articles on COL5A1

1. Col5a1 knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with COL5A1 elisa kit

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with COL5A1 elisa kit

Disease Name	Pubmed Publications
Ehlers-Danlos syndrome type 2 Antibodies	>24 publications with COL5A1 and Ehlers-Danlos syndrome type 2
Ehlers-Danlos syndrome type 1 Antibodies	>23 publications with COL5A1 and Ehlers-Danlos syndrome type 1
Disease Models, Animal Antibodies	>10 publications with COL5A1 and Disease Models, Animal
Fibrosis Antibodies	>6 publications with COL5A1 and Fibrosis
Inflammation Antibodies	>6 publications with COL5A1 and Inflammation
Carcinoma Antibodies	>3 publications with COL5A1 and Carcinoma
Heart Diseases Antibodies	>3 publications with COL5A1 and Heart Diseases
Necrosis Antibodies	>2 publications with COL5A1 and Necrosis
Kidney Diseases Antibodies	>2 publications with COL5A1 and Kidney Diseases
Hemorrhage Antibodies	>2 publications with COL5A1 and Hemorrhage

Organs/Tissues associated with COL5A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>34 publications with COL5A1 and Connective Tissue
Joint Antibodies	>31 publications with COL5A1 and Joint
Skin Antibodies	>29 publications with COL5A1 and Skin
Bone Antibodies	>16 publications with COL5A1 and Bone
Eye Antibodies	>9 publications with COL5A1 and Eye
Muscle Antibodies	>8 publications with COL5A1 and Muscle
Embryonic Tissue Antibodies	>6 publications with COL5A1 and Embryonic Tissue
Brain Antibodies	>3 publications with COL5A1 and Brain
Kidney Antibodies	>3 publications with COL5A1 and Kidney
Lung Antibodies	>3 publications with COL5A1 and Lung