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Integrin Alpha-M (Itgam/Cd11b) (ITGAM/CD11b) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS934157

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Itgam/CD11b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Itgam/CD11b present is bound… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Integrin alpha-M (Itgam/CD11b), ELISA Kit
Full Product Name
Mouse Integrin alpha-M, Itgam/CD11b ELISA Kit
Product Gene Name
Itgam/CD11b elisa kit
Product Synonym Names
Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit; CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6; antigen CD11b (p170) ; integrin alpha M; macrophage antigen alpha polypeptide; neutrophil adherence receptor alpha-M subunit; Integrin alpha-M (Itgam/CD11b)
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse Itgam/CD11b. No significant cross-reactivity or interference between mouse Itgam/CD11b and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
127,481 Da
3D Structure
ModBase 3D Structure for P05555
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of Itgam/CD11b elisa kit

Mouse ITGAM/CD11b ELISA Kit – Cat# MBS934157

Other Notes

Small volumes of Itgam/CD11b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Itgam/CD11b purchase

MBS934157 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Integrin alpha-M (Itgam/CD11b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Itgam/CD11b. The ELISA analytical biochemical technique of the MBS934157 kit is based on Itgam/CD11b antibody-Itgam/CD11b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Itgam/CD11b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Itgam/CD11b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Itgam/CD11b. It may not necessarily be applicable to this product.

NCBI GI #
124956
NCBI GeneID
16409
NCBI Accession #
P05555.2
UniProt Primary Accession #
P05555
UniProt Secondary Accession #
Q8CA73
UniProt Related Accession #
P05555
NCBI Official Full Name
Integrin alpha-M
NCBI Official Synonym Full Names
integrin alpha M
NCBI Official Symbol
Itgam
NCBI Official Synonym Symbols
CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
UniProt Protein Name
Integrin alpha-M
UniProt Synonym Protein Names
CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; CD_antigen: CD11b
UniProt Gene Name
Itgam
UniProt Entry Name
ITAM_MOUSE
NCBI Protein Information
integrin alpha-M; Mac-1 alpha; CD11B (p170); CR-3 alpha chain; macrophage antigen alpha; complement receptor type 3; leukocyte adhesion receptor MO1; CD11 antigen-like family member B; complement component receptor 3 alpha-a; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha

UniProt Comments for Itgam/CD11b

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Cellular Component: cell surface; membrane; integral to membrane; integrin complex; nucleus; external side of plasma membrane

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; cell-cell adhesion; leukocyte adhesion; microglia development; cellular extravasation; activated T cell proliferation; cell adhesion; leukocyte migration during inflammatory response
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Research Articles on Itgam/CD11b

1. CD103-CD11b+ dendritic cells regulate the sensitivity of CD4 T-cell responses to bacterial flagellin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Itgam/CD11b elisa kit

 Reference Product  PubMed Publications
 Itgb2 elisa kit  >1565 publications with Itgam/CD11b and Itgb2

Pathways associated with Itgam/CD11b elisa kit

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram

Organs/Tissues associated with Itgam/CD11b elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3890 publications with Itgam/CD11b and Blood
 Bone Marrow Antibodies  >634 publications with Itgam/CD11b and Bone Marrow
 Brain Antibodies  >445 publications with Itgam/CD11b and Brain
 Lung Antibodies  >407 publications with Itgam/CD11b and Lung
 Spleen Antibodies  >398 publications with Itgam/CD11b and Spleen
 Skin Antibodies  >206 publications with Itgam/CD11b and Skin
 Heart Antibodies  >152 publications with Itgam/CD11b and Heart
 Spinal Cord Antibodies  >128 publications with Itgam/CD11b and Spinal Cord
 Intestine Antibodies  >125 publications with Itgam/CD11b and Intestine
 Eye Antibodies  >123 publications with Itgam/CD11b and Eye
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