• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Ezrin (EZR) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS934429

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EZR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EZR present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ezrin, ELISA Kit
Full Product Name
Mouse Ezrin, EZR ELISA Kit
Product Gene Name
EZR elisa kit
Product Synonym Names
Mouse Ezrin (EZR) ELISA kit; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584; VIL2; cytovillin 2; villin 2 (ezrin) ; ezrin
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
62.5 pg/ml -4000 pg/ml.
Sensitivity
The minimum detectable dose of mouse EZR is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse EZR. No significant cross-reactivity or interference between mouse EZR and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
69,407 Da
3D Structure
ModBase 3D Structure for P26040
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of EZR elisa kit

Mouse EZR ELISA Kit – Cat# MBS934429

Other Notes

Small volumes of EZR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EZR purchase

MBS934429 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ezrin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EZR. The ELISA analytical biochemical technique of the MBS934429 kit is based on EZR antibody-EZR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EZR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EZR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EZR. It may not necessarily be applicable to this product.

NCBI GI #
83921618
NCBI GeneID
22350
NCBI Accession #
NP_033536.2
UniProt Primary Accession #
P26040
UniProt Secondary Accession #
Q80ZT8; Q9DCI1
UniProt Related Accession #
P26040
GenBank Nucleotide #
NM_009510.2
NCBI Official Full Name
ezrin
NCBI Official Synonym Full Names
ezrin
NCBI Official Symbol
Ezr
NCBI Official Synonym Symbols
p81; Vil2; R75297; AW146364
UniProt Protein Name
Ezrin
UniProt Synonym Protein Names
Cytovillin; Villin-2; p81
Protein Family
Ezrin
UniProt Gene Name
Ezr
UniProt Synonym Gene Names
Vil2
UniProt Entry Name
EZRI_MOUSE
NCBI Protein Information
ezrin; villin 2; villin-2; cytovillin

UniProt Comments for EZR

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; T-tubule; cytosol; lipid raft; membrane; apical plasma membrane; cytoplasm; nucleolus; intracellular; uropod; vesicle; myelin sheath; filopodium; focal adhesion; actin filament; actin cytoskeleton; ruffle; extrinsic to membrane; cell projection; cytoskeleton; microvillus membrane; apical part of cell; plasma membrane

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cytoskeletal protein binding; protein complex binding; cell adhesion molecule binding; actin binding

Biological Process: actin filament bundle formation; regulation of cell shape; microvillus biogenesis; filopodium formation; receptor internalization; establishment and/or maintenance of apical/basal cell polarity
Show more

Research Articles on EZR

1. ALCAM stably interacts with actin by binding to syntenin-1 and ezrin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EZR elisa kit

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 L1CAM Interactions Pathway antibodies  L1CAM Interactions Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
 Recycling Pathway Of L1 antibodies  Recycling Pathway Of L1 Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with EZR elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with EZR and Neoplasms
 Disease Models, Animal Antibodies  >1 publications with EZR and Disease Models, Animal
 Inflammation Antibodies  >1 publications with EZR and Inflammation

Organs/Tissues associated with EZR elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lymph Node Antibodies  >1 publications with EZR and Lymph Node
 Blood Antibodies  >1 publications with EZR and Blood
 Eye Antibodies  >1 publications with EZR and Eye
 Bone Antibodies  >1 publications with EZR and Bone
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000