Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) ELISA Kit for Human

Name: Human HAO1 ELISA Kit; Hydroxyacid Oxidase (Glycolate Oxidase) 1; MyBioSource
Brand: MyBioSource
SKU: MBS934775
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS934775

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAO1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

hydroxyacid oxidase (glycolate oxidase) 1 (HAO1), ELISA Kit

Full Product Name

Human Hydroxyacid oxidase 1, HAO1 ELISA Kit

Product Gene Name

HAO1 elisa kit

Product Synonym Names

Human Hydroxyacid oxidase 1 (HAO1) ELISA kit; GOX; GOX1; HAOX1; MGC142225; MGC142227; (S) -2-hydroxy-acid oxidase; glycolate oxidase; hydroxyacid oxidase 1; hydroxyacid oxidase (glycolate oxidase) 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

serum, plasma, tissue homogenates, cell lysates

Detection Range

18.75 pg/ml -1200 pg/ml

Sensitivity

<4.69 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human HAO1. No significant cross-reactivity or interference between human HAO1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

40,924 Da

OMIM

605023

3D Structure

ModBase 3D Structure for Q9UJM8

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HAO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAO1 purchase

MBS934775 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hydroxyacid oxidase (glycolate oxidase) 1 (HAO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HAO1. The ELISA analytical biochemical technique of the MBS934775 kit is based on HAO1 antibody-HAO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HAO1. It may not necessarily be applicable to this product.

NCBI GI #

11068137

NCBI GeneID

54363

NCBI Accession #

NP_060015.1

UniProt Primary Accession #

Q9UJM8

UniProt Secondary Accession #

Q14CQ0; Q9UPZ0; Q9Y3I7

UniProt Related Accession #

Q9UJM8

GenBank Nucleotide #

NM_017545.2

NCBI Official Full Name

hydroxyacid oxidase 1

NCBI Official Synonym Full Names

hydroxyacid oxidase (glycolate oxidase) 1

NCBI Official Symbol

HAO1

NCBI Official Synonym Symbols

GOX; GOX1; HAOX1

UniProt Protein Name

Hydroxyacid oxidase 1

UniProt Synonym Protein Names

Glycolate oxidase; GOX

Protein Family

Hydroxyacid oxidase

UniProt Gene Name

HAO1

UniProt Synonym Gene Names

GOX1; HAOX1; HAOX1; GOX

UniProt Entry Name

HAOX1_HUMAN

NCBI Protein Information

hydroxyacid oxidase 1; glycolate oxidase; (S)-2-hydroxy-acid oxidase

NCBI Summary for HAO1

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]

UniProt Comments for HAO1

HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Oxidoreductase; EC 1.1.3.15

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: peroxisomal matrix; peroxisome

Molecular Function: glycolate oxidase activity; FMN binding; glyoxylate oxidase activity; receptor binding; (S)-2-hydroxy-acid oxidase activity

Biological Process: glyoxylate metabolic process; response to oxidative stress; glycolate catabolic process; fatty acid alpha-oxidation

Research Articles on HAO1

1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HAO1 elisa kit

Products by Pathway	Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate Metabolism Pathway antibodies	Glyoxylate Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Peroxisome Pathway antibodies	Peroxisome Pathway Diagram
Peroxisome Pathway antibodies	Peroxisome Pathway Diagram

Diseases associated with HAO1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1 publications with HAO1 and Neoplasms
Fatty Liver Antibodies	>1 publications with HAO1 and Fatty Liver

Organs/Tissues associated with HAO1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with HAO1 and Liver
Kidney Antibodies	>1 publications with HAO1 and Kidney
Pancreas Antibodies	>1 publications with HAO1 and Pancreas