Complement Component 6 (C6) ELISA Kit for Human

Name: Human C6 ELISA Kit; Complement Component 6; MyBioSource
Brand: MyBioSource
SKU: MBS934887
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS934887

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C6 present is bound by the immobilized… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

complement component 6 (C6), ELISA Kit

Full Product Name

Human Complement component C6, C6 ELISA Kit

Product Gene Name

C6 elisa kit

Product Synonym Names

Human Complement component C6 (C6) ELISA kit; complement component 6

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

62.5 pg/ml -4000 pg/ml.

Sensitivity

The minimum detectable dose of human C6 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human C6. No significant cross-reactivity or interference between human C6 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

104,786 Da

OMIM

gene 612446

3D Structure

ModBase 3D Structure for P13671

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of C6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C6 purchase

MBS934887 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the complement component 6 (C6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C6. The ELISA analytical biochemical technique of the MBS934887 kit is based on C6 antibody-C6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for C6. It may not necessarily be applicable to this product.

NCBI GI #

169636415

NCBI GeneID

729

NCBI Accession #

NP_000056.2

UniProt Primary Accession #

P13671

UniProt Related Accession #

P13671

GenBank Nucleotide #

NM_000065.3

NCBI Official Full Name

complement component C6

NCBI Official Synonym Full Names

complement component 6

NCBI Official Symbol

UniProt Protein Name

Complement component C6

Protein Family

Complement component

UniProt Gene Name

UniProt Entry Name

CO6_HUMAN

NCBI Protein Information

complement component C6

NCBI Summary for C6

This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]

UniProt Comments for C6

C6: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Molecular Function: protein binding

Biological Process: positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; in utero embryonic development; cytolysis; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 6 Deficiency

Research Articles on C6

1. Crystal structure of C5b-6 suggests structural basis for priming assembly of the membrane attack complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with C6 elisa kit

Products by Pathway	Pathway Diagram
Complement Activation, Classical Pathway antibodies	Complement Activation, Classical Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement Cascade Pathway antibodies	Complement Cascade Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Prion Diseases Pathway antibodies	Prion Diseases Pathway Diagram
Prion Diseases Pathway antibodies	Prion Diseases Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram

Diseases associated with C6 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3760 publications with C6 and Neoplasms
Nervous System Diseases Antibodies	>2774 publications with C6 and Nervous System Diseases
Brain Diseases Antibodies	>1342 publications with C6 and Brain Diseases
Pain Antibodies	>668 publications with C6 and Pain
Disease Models, Animal Antibodies	>460 publications with C6 and Disease Models, Animal
Drug Toxicity Antibodies	>457 publications with C6 and Drug Toxicity
Necrosis Antibodies	>359 publications with C6 and Necrosis
COMPLEMENT COMPONENT 6 DEFICIENCY Antibodies	>297 publications with C6 and COMPLEMENT COMPONENT 6 DEFICIENCY
Postoperative Complications Antibodies	>254 publications with C6 and Postoperative Complications
Inflammation Antibodies	>243 publications with C6 and Inflammation

Organs/Tissues associated with C6 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2492 publications with C6 and Brain
Liver Antibodies	>461 publications with C6 and Liver
Kidney Antibodies	>361 publications with C6 and Kidney
Heart Antibodies	>282 publications with C6 and Heart
Lung Antibodies	>225 publications with C6 and Lung
Connective Tissue Antibodies	>85 publications with C6 and Connective Tissue
Bladder Antibodies	>68 publications with C6 and Bladder
Ovary Antibodies	>58 publications with C6 and Ovary
Trachea Antibodies	>36 publications with C6 and Trachea
Testis Antibodies	>35 publications with C6 and Testis