Chordin-Like 1 (CHRDL1) ELISA Kit for Rat

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRDL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRDL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of CHRDL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS935592 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the chordin-like 1 (CHRDL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHRDL1. The ELISA analytical biochemical technique of the MBS935592 kit is based on CHRDL1 antibody-CHRDL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRDL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRDL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). With questionable cellular roles and apparent antagonism for embryonic receptor types, Chordin-like protein 1 (CHRDL) is an active area of investigation in developmental biology. We offer a highly rat species specific CHRDL1 ELISA to assay this protein with minimal cross-reactivity to other transforming growth factor - TGF beta superfamily - members, like bone morphogenetic protein (BMP) or chordin protein 1. Our ELISA kit demonstrates ng/ml levels of detection in a sandwich style format and comes complete with antibody prebound 1 plate for rapid quantitation of samples, using MBS substrates and all the information required for success detailed on the datasheet. Human and mouse ELISA kits, along with many related and potentially crucial proteins in stem cell experiments, typified by ELISA kit cholinergic receptor nicotinic, gt transforming growth factor beta and chordin 1 ELISA kit, are readily available with our same high quality standards, value pricing and unexpected services.