Leptin (LEP) ELISA Kit for Horse

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LEP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LEP. The competitive inhibition reaction is launched between with pre-coated LEP and LEP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LEP in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of LEP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS936352 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leptin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LEP. The ELISA analytical biochemical technique of the MBS936352 kit is based on LEP antibody-LEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).