Jagged 1 (Alagille Syndrome) (JAG1) ELISA Kit for Human

Name: Human JAG1 ELISA Kit; Jagged 1 (Alagille Syndrome); MyBioSource
Brand: MyBioSource
SKU: MBS936684
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS936684

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for JAG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any JAG1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

jagged 1 (Alagille syndrome) (JAG1), ELISA Kit

Full Product Name

Human Protein jagged-1, JAG1 ELISA Kit

Product Gene Name

JAG1 elisa kit

Product Synonym Names

Human Protein jagged-1 (JAG1) ELISA kit; AGS; AHD; AWS; CD339; HJ1; JAGL1; MGC104644; jagged 1; jagged 1 (Alagille syndrome)

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates.

Detection Range

0.156 ng/ml -10 ng/ml.

Sensitivity

0.039 ng/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human JAG1. No significant cross-reactivity or interference between human JAG1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

133,799 Da

OMIM

601920

3D Structure

ModBase 3D Structure for P78504

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of JAG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JAG1 purchase

MBS936684 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the jagged 1 (Alagille syndrome) (JAG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing JAG1. The ELISA analytical biochemical technique of the MBS936684 kit is based on JAG1 antibody-JAG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect JAG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, JAG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for JAG1. It may not necessarily be applicable to this product.

NCBI GI #

4557679

NCBI GeneID

182

NCBI Accession #

NP_000205.1

UniProt Primary Accession #

P78504

UniProt Secondary Accession #

O14902; O15122; Q15816; A0AV43

UniProt Related Accession #

P78504

GenBank Nucleotide #

NM_000214.2

NCBI Official Full Name

protein jagged-1

NCBI Official Synonym Full Names

jagged 1

NCBI Official Symbol

JAG1

NCBI Official Synonym Symbols

AGS; AHD; AWS; HJ1; CD339; JAGL1

UniProt Protein Name

Protein jagged-1

UniProt Synonym Protein Names

CD_antigen: CD339

Protein Family

Protein jagged

UniProt Gene Name

JAG1

UniProt Synonym Gene Names

JAGL1; Jagged1; hJ1

UniProt Entry Name

JAG1_HUMAN

NCBI Protein Information

protein jagged-1

NCBI Summary for JAG1

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]

UniProt Comments for JAG1

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.1-p11.23

Cellular Component: membrane; integral to plasma membrane; apical part of cell; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding; structural molecule activity

Biological Process: nervous system development; endothelial cell differentiation; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; keratinocyte differentiation; myoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; hemopoiesis; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of Notch signaling pathway

Disease: Tetralogy Of Fallot; Alagille Syndrome 1

Research Articles on JAG1

1. the N terminus of human Jagged-1 is a C2 phospholipid recognition domain that binds phospholipid bilayers in a calcium-dependent fashion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with JAG1 elisa kit

Reference Product	PubMed Publications
NOTCH1 elisa kit	>49 publications with JAG1 and NOTCH1
NOTCH2 elisa kit	>34 publications with JAG1 and NOTCH2
NOTCH3 elisa kit	>21 publications with JAG1 and NOTCH3
MFNG elisa kit	>4 publications with JAG1 and MFNG
JUN elisa kit	>1 publications with JAG1 and JUN
FOS elisa kit	>1 publications with JAG1 and FOS

Pathways associated with JAG1 elisa kit

Products by Pathway	Pathway Diagram
Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies	Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
Notch Signaling Pathway antibodies	Notch Signaling Pathway Diagram
Notch Signaling Pathway antibodies	Notch Signaling Pathway Diagram
Notch Signaling Pathway antibodies	Notch Signaling Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By NOTCH Pathway antibodies	Signaling By NOTCH Pathway Diagram
Signaling By NOTCH1 Pathway antibodies	Signaling By NOTCH1 Pathway Diagram
Signaling By NOTCH2 Pathway antibodies	Signaling By NOTCH2 Pathway Diagram
Signaling By NOTCH3 Pathway antibodies	Signaling By NOTCH3 Pathway Diagram

Diseases associated with JAG1 elisa kit

Disease Name	Pubmed Publications
Alagille Syndrome Antibodies	>112 publications with JAG1 and Alagille Syndrome
Congenital Abnormalities Antibodies	>104 publications with JAG1 and Congenital Abnormalities
Cardiovascular Diseases Antibodies	>103 publications with JAG1 and Cardiovascular Diseases
Heart Defects, Congenital Antibodies	>99 publications with JAG1 and Heart Defects, Congenital
Liver Diseases Antibodies	>95 publications with JAG1 and Liver Diseases
Neoplasms Antibodies	>39 publications with JAG1 and Neoplasms
Facies Antibodies	>28 publications with JAG1 and Facies
Heart Diseases Antibodies	>24 publications with JAG1 and Heart Diseases
Carcinoma Antibodies	>16 publications with JAG1 and Carcinoma
Breast Neoplasms Antibodies	>16 publications with JAG1 and Breast Neoplasms

Organs/Tissues associated with JAG1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>57 publications with JAG1 and Liver
Heart Antibodies	>41 publications with JAG1 and Heart
Vascular Antibodies	>29 publications with JAG1 and Vascular
Eye Antibodies	>25 publications with JAG1 and Eye
Blood Antibodies	>25 publications with JAG1 and Blood
Embryonic Tissue Antibodies	>20 publications with JAG1 and Embryonic Tissue
Ear Antibodies	>19 publications with JAG1 and Ear
Kidney Antibodies	>15 publications with JAG1 and Kidney
Brain Antibodies	>13 publications with JAG1 and Brain
Muscle Antibodies	>10 publications with JAG1 and Muscle