Thrombospondin 4 (THBS4) ELISA Kit for Human

Name: Human THBS4 ELISA Kit; Thrombospondin 4; MyBioSource
Brand: MyBioSource
SKU: MBS937065
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS937065

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for THBS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any THBS4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

thrombospondin 4 (THBS4), ELISA Kit

Full Product Name

Human Thrombospondin-4, THBS4 ELISA Kit

Product Gene Name

THBS4 elisa kit

Product Synonym Names

Human Thrombospondin-4 (THBS4) ELISA kit; TSP4; ; thrombospondin 4

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma

Detection Range

7.8 ng/ml -500 ng/ml.

Sensitivity

The minimum detectable dose of human THBS4 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human THBS4. No significant cross-reactivity or interference between human THBS4 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

105,869 Da

OMIM

600715

3D Structure

ModBase 3D Structure for P35443

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of THBS4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for THBS4 purchase

MBS937065 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the thrombospondin 4 (THBS4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing THBS4. The ELISA analytical biochemical technique of the MBS937065 kit is based on THBS4 antibody-THBS4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect THBS4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, THBS4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for THBS4. It may not necessarily be applicable to this product.

NCBI GI #

31543806

NCBI GeneID

7060

NCBI Accession #

NP_003239.2

UniProt Primary Accession #

P35443

UniProt Secondary Accession #

Q86TG2; B2R909

UniProt Related Accession #

P35443

GenBank Nucleotide #

NM_003248.4

NCBI Official Full Name

thrombospondin-4

NCBI Official Synonym Full Names

thrombospondin 4

NCBI Official Symbol

THBS4

NCBI Official Synonym Symbols

TSP4

UniProt Protein Name

Thrombospondin-4

Protein Family

Thrombospondin

UniProt Gene Name

THBS4

UniProt Synonym Gene Names

TSP4

UniProt Entry Name

TSP4_HUMAN

NCBI Protein Information

thrombospondin-4

NCBI Summary for THBS4

The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Studies of the rat counterpart suggest that this protein may be involved in local signaling in the developing and adult nervous system. [provided by RefSeq, Jul 2008]

UniProt Comments for THBS4

THBS4: Acts as an extracellular mitogen and stimulates early erythroid progenitors proliferation in presence of EPO. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: extracellular matrix; extracellular space; sarcoplasmic reticulum; endoplasmic reticulum; extracellular region; basement membrane

Molecular Function: heparin binding; integrin binding; protein binding; growth factor activity; calcium ion binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; behavioral response to pain; negative regulation of angiogenesis; positive regulation of cell division; myoblast migration; tissue remodeling; positive regulation of endothelial cell proliferation; response to unfolded protein; regulation of tissue remodeling

Research Articles on THBS4

1. THBS4 expression in breast cancer-associated extracellular matrix contributes to the activated stromal response exhibited during tumour progression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with THBS4 elisa kit

Products by Pathway	Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Phagosome Pathway antibodies	Phagosome Pathway Diagram
Phagosome Pathway antibodies	Phagosome Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with THBS4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>4 publications with THBS4 and Neoplasms
Hypertension Antibodies	>3 publications with THBS4 and Hypertension
Heart Diseases Antibodies	>3 publications with THBS4 and Heart Diseases
Myocardial Ischemia Antibodies	>3 publications with THBS4 and Myocardial Ischemia
Inflammation Antibodies	>2 publications with THBS4 and Inflammation
Liver Neoplasms Antibodies	>1 publications with THBS4 and Liver Neoplasms
Liver Diseases Antibodies	>1 publications with THBS4 and Liver Diseases
Skin Abnormalities Antibodies	>1 publications with THBS4 and Skin Abnormalities
Vasculitis Antibodies	>1 publications with THBS4 and Vasculitis
Disease Models, Animal Antibodies	>1 publications with THBS4 and Disease Models, Animal

Organs/Tissues associated with THBS4 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>3 publications with THBS4 and Bone
Heart Antibodies	>3 publications with THBS4 and Heart
Blood Antibodies	>2 publications with THBS4 and Blood
Kidney Antibodies	>2 publications with THBS4 and Kidney
Brain Antibodies	>2 publications with THBS4 and Brain
Nerve Antibodies	>1 publications with THBS4 and Nerve
Intestine Antibodies	>1 publications with THBS4 and Intestine
Muscle Antibodies	>1 publications with THBS4 and Muscle
Stomach Antibodies	>1 publications with THBS4 and Stomach