Agouti Related Protein Homolog (Mouse) (AGRP) ELISA Kit for Mouse

Name: Mouse AGRP ELISA Kit; Agouti Related Protein Homolog (Mouse); MyBioSource
Brand: MyBioSource
SKU: MBS937696
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS937696

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGRP present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

agouti related protein homolog (mouse) (AGRP), ELISA Kit

Full Product Name

Mouse Agouti-related protein, AGRP ELISA Kit

Product Gene Name

AGRP elisa kit

Product Synonym Names

Mouse Agouti-related protein (AGRP) ELISA kit; AGRT; ART; ASIP2; MGC118963; agouti related protein; agouti related protein homolog (mouse)

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

15.6 pg/ml-1000 pg/ml.

Sensitivity

The minimum detectable dose of mouse AGRP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse AGRP. No significant cross-reactivity or interference between mouse AGRP and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

14,432 Da

3D Structure

ModBase 3D Structure for P56473

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of AGRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AGRP purchase

MBS937696 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the agouti related protein homolog (mouse) (AGRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGRP. The ELISA analytical biochemical technique of the MBS937696 kit is based on AGRP antibody-AGRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AGRP. It may not necessarily be applicable to this product.

NCBI GI #

424036681

NCBI GeneID

11604

NCBI Accession #

NP_001258735.1

UniProt Primary Accession #

P56473

UniProt Secondary Accession #

O35967

UniProt Related Accession #

P56473

GenBank Nucleotide #

NM_001271806.1

NCBI Official Full Name

agouti-related protein

NCBI Official Synonym Full Names

agouti related protein

NCBI Official Symbol

Agrp

NCBI Official Synonym Symbols

Art; Agrt

UniProt Protein Name

Agouti-related protein

Protein Family

Agouti-related protein

UniProt Gene Name

Agrp

UniProt Synonym Gene Names

Agrt; Art

UniProt Entry Name

AGRP_MOUSE

NCBI Protein Information

agouti-related protein; orexigenic neuropeptide

NCBI Summary for AGRP

This gene encodes a protein that regulates feeding behavior and plays a key role in the control of body weight. The encoded protein acts as an antagonist of melanocortin receptor signaling. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Nov 2012]

UniProt Comments for AGRP

AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Protein type: Secreted, signal peptide; Hormone; Secreted

Cellular Component: Golgi apparatus; extracellular space; cell soma; Golgi lumen; extracellular region

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: hormone-mediated signaling; eating behavior; neuropeptide signaling pathway; adult feeding behavior

Disease: Obesity

Research Articles on AGRP

1. Inhibitors of NOS and the cystic fibrosis transmembrane conductance regulator (CFTR) prevented a decrease in AgRP secretion with glucose, suggesting a pivotal role for nNOS and the CFTR in glucose-sensing.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with AGRP elisa kit

Products by Pathway	Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram

Diseases associated with AGRP elisa kit

Disease Name	Pubmed Publications
Obesity Antibodies	>288 publications with AGRP and Obesity
Weight Gain Antibodies	>87 publications with AGRP and Weight Gain
Weight Loss Antibodies	>72 publications with AGRP and Weight Loss
Disease Models, Animal Antibodies	>47 publications with AGRP and Disease Models, Animal
Mental Disorders Antibodies	>27 publications with AGRP and Mental Disorders
Hyperglycemia Antibodies	>20 publications with AGRP and Hyperglycemia
Inflammation Antibodies	>14 publications with AGRP and Inflammation
Nervous System Diseases Antibodies	>10 publications with AGRP and Nervous System Diseases
Neoplasms Antibodies	>10 publications with AGRP and Neoplasms
Diabetes Mellitus, Type 2 Antibodies	>7 publications with AGRP and Diabetes Mellitus, Type 2

Organs/Tissues associated with AGRP elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>526 publications with AGRP and Brain
Eye Antibodies	>4 publications with AGRP and Eye