Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN) ELISA Kit for Mouse

Name: Mouse CUBN ELISA Kit; Cubilin (Intrinsic Factor-Cobalamin Receptor); MyBioSource
Brand: MyBioSource
SKU: MBS939514
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS939514

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CUBN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CUBN present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

cubilin (intrinsic factor-cobalamin receptor) (CUBN), ELISA Kit

Full Product Name

Mouse Cubilin, CUBN ELISA Kit

Product Gene Name

CUBN elisa kit

Product Synonym Names

Mouse Cubilin (CUBN) ELISA kit; RP11-416D8.1; FLJ90055; FLJ90747; IFCR; MGA1; gp280; cubilin; intrinsic factor B12-receptor; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; cubilin (intrinsic factor-cobalamin receptor)

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates.

Detection Range

7.81 pg/ml-500 pg/ml.

Sensitivity

The minimum detectable dose of mouse CUBN is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse CUBN. No significant cross-reactivity or interference between mouse CUBN and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

399,097 Da

3D Structure

ModBase 3D Structure for Q9JLB4

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CUBN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CUBN purchase

MBS939514 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cubilin (intrinsic factor-cobalamin receptor) (CUBN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CUBN. The ELISA analytical biochemical technique of the MBS939514 kit is based on CUBN antibody-CUBN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CUBN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CUBN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CUBN. It may not necessarily be applicable to this product.

NCBI GI #

124487348

NCBI GeneID

65969

NCBI Accession #

NP_001074553.1

UniProt Primary Accession #

Q9JLB4

UniProt Secondary Accession #

B1AX10

UniProt Related Accession #

Q9JLB4

GenBank Nucleotide #

NM_001081084.2

NCBI Official Full Name

cubilin

NCBI Official Synonym Full Names

cubilin (intrinsic factor-cobalamin receptor)

NCBI Official Symbol

Cubn

NCBI Official Synonym Symbols

AA408369; AL022750; D2Wsu88e

UniProt Protein Name

Cubilin

UniProt Synonym Protein Names

Intrinsic factor-cobalamin receptor

Protein Family

Cubilin

UniProt Gene Name

Cubn

UniProt Synonym Gene Names

Ifcr

UniProt Entry Name

CUBN_MOUSE

NCBI Protein Information

cubilin; intrinsic factor-cobalamin receptor

UniProt Comments for CUBN

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.

Cellular Component: Golgi apparatus; lysosomal lumen; protein complex; brush border membrane; endoplasmic reticulum; lysosome; coated pit; membrane; apical part of cell; endocytic vesicle; apical plasma membrane; cytoplasm; brush border; endosome

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; cobalamin transporter activity; hemoglobin binding; metal ion binding; receptor activity; calcium ion binding; cobalamin binding

Biological Process: steroid metabolic process; cholesterol metabolic process; receptor-mediated endocytosis; protein transport; in utero embryonic development; transport; lipoprotein transport; cobalamin transport; hemoglobin import; lipid metabolic process; response to nutrient

Research Articles on CUBN

1. Cubilin deficiency reduces renal salvage and delivery back to the blood of albumin and apolipoprotein (apo)A-I, which decreases blood levels of albumin and apoA-I/HD

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CUBN elisa kit

Products by Pathway	Pathway Diagram
HDL-mediated Lipid Transport Pathway antibodies	HDL-mediated Lipid Transport Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Lipoprotein Metabolism Pathway antibodies	Lipoprotein Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Steroid Hormones And Vitamins A And D Pathway antibodies	Metabolism Of Steroid Hormones And Vitamins A And D Pathway Diagram
PluriNetWork Pathway antibodies	PluriNetWork Pathway Diagram
Vitamin D (calciferol) Metabolism Pathway antibodies	Vitamin D (calciferol) Metabolism Pathway Diagram
Vitamin Digestion And Absorption Pathway antibodies	Vitamin Digestion And Absorption Pathway Diagram
Vitamin Digestion And Absorption Pathway antibodies	Vitamin Digestion And Absorption Pathway Diagram

Diseases associated with CUBN elisa kit

Disease Name	Pubmed Publications
Proteinuria Antibodies	>14 publications with CUBN and Proteinuria
Anemia, Megaloblastic Antibodies	>11 publications with CUBN and Anemia, Megaloblastic
Kidney Diseases Antibodies	>2 publications with CUBN and Kidney Diseases
Necrosis Antibodies	>1 publications with CUBN and Necrosis
Inflammation Antibodies	>1 publications with CUBN and Inflammation
Renal Insufficiency Antibodies	>1 publications with CUBN and Renal Insufficiency

Organs/Tissues associated with CUBN elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>7 publications with CUBN and Kidney
Eye Antibodies	>1 publications with CUBN and Eye
Brain Antibodies	>1 publications with CUBN and Brain
Embryonic Tissue Antibodies	>1 publications with CUBN and Embryonic Tissue