Y Box Binding Protein 1 (YBX1) ELISA Kit for Human

Name: Human YBX1 ELISA Kit; Y Box Binding Protein 1; MyBioSource
Brand: MyBioSource
SKU: MBS939527
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS939527

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YBX1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Y box binding protein 1 (YBX1), ELISA Kit

Full Product Name

Human Nuclease-sensitive element-binding protein 1, YBX1 ELISA Kit

Product Gene Name

YBX1 elisa kit

Product Synonym Names

Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA kit; BP-8; CSDA2; CSDB; DBPB; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP-1; NSEP1; YB-1; YB1; DNA-binding protein B; major histocompatibility complex; class II; Y box-binding protein I; nuclease sensit; Y box binding protein 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/ml-2000 pg/ml

Sensitivity

< 7.81 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human YBX1. No significant cross-reactivity or interference between human YBX1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%.Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

35,924 Da

OMIM

154030

3D Structure

ModBase 3D Structure for P67809

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of YBX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YBX1 purchase

MBS939527 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Y box binding protein 1 (YBX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YBX1. The ELISA analytical biochemical technique of the MBS939527 kit is based on YBX1 antibody-YBX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YBX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YBX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for YBX1. It may not necessarily be applicable to this product.

NCBI GI #

34098946

NCBI GeneID

4904

NCBI Accession #

NP_004550.2

UniProt Primary Accession #

P67809

UniProt Secondary Accession #

P16990; P16991; Q14972; Q15325; Q5FVF0

UniProt Related Accession #

P67809

GenBank Nucleotide #

NM_004559.3

NCBI Official Full Name

nuclease-sensitive element-binding protein 1

NCBI Official Synonym Full Names

Y box binding protein 1

NCBI Official Symbol

YBX1

NCBI Official Synonym Symbols

YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1

UniProt Protein Name

Nuclease-sensitive element-binding protein 1

UniProt Synonym Protein Names

CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1

Protein Family

Nuclease-sensitive element-binding protein

UniProt Gene Name

YBX1

UniProt Synonym Gene Names

NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1

UniProt Entry Name

YBOX1_HUMAN

NCBI Protein Information

nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I

UniProt Comments for YBX1

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.

Protein type: RNA processing; Transcription factor; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter

Research Articles on YBX1

1. Plasma YB-1/p18 detection has a high specific prevalence in malignancies, thereby providing a novel tool for cancer screening independent of the tumor origin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with YBX1 elisa kit

Reference Product	PubMed Publications
TFAP2A elisa kit	>7 publications with YBX1 and TFAP2A
tat elisa kit	>5 publications with YBX1 and tat
PCNA elisa kit	>4 publications with YBX1 and PCNA
PURA elisa kit	>4 publications with YBX1 and PURA
SMAD3 elisa kit	>2 publications with YBX1 and SMAD3
FUS elisa kit	>2 publications with YBX1 and FUS
TP53 elisa kit	>2 publications with YBX1 and TP53
CTCF elisa kit	>2 publications with YBX1 and CTCF
SLC6A4 elisa kit	>1 publications with YBX1 and SLC6A4
NFYB elisa kit	>1 publications with YBX1 and NFYB

Pathways associated with YBX1 elisa kit

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
IL-2 Signaling Pathway antibodies	IL-2 Signaling Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
SIDS Susceptibility Pathways antibodies	SIDS Susceptibility Pathways Diagram
Signaling Events Mediated By PTP1B Pathway antibodies	Signaling Events Mediated By PTP1B Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram
MRNA Processing Pathway antibodies	MRNA Processing Pathway Diagram

Diseases associated with YBX1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>122 publications with YBX1 and Neoplasms
Carcinoma Antibodies	>39 publications with YBX1 and Carcinoma
Breast Neoplasms Antibodies	>39 publications with YBX1 and Breast Neoplasms
Liver Diseases Antibodies	>15 publications with YBX1 and Liver Diseases
Adenocarcinoma Antibodies	>14 publications with YBX1 and Adenocarcinoma
Lung Neoplasms Antibodies	>11 publications with YBX1 and Lung Neoplasms
Liver Neoplasms Antibodies	>10 publications with YBX1 and Liver Neoplasms
Disease Models, Animal Antibodies	>9 publications with YBX1 and Disease Models, Animal
Chromosome Aberrations Antibodies	>8 publications with YBX1 and Chromosome Aberrations
Neoplasm Metastasis Antibodies	>8 publications with YBX1 and Neoplasm Metastasis

Organs/Tissues associated with YBX1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>83 publications with YBX1 and Liver
Brain Antibodies	>36 publications with YBX1 and Brain
Blood Antibodies	>29 publications with YBX1 and Blood
Kidney Antibodies	>25 publications with YBX1 and Kidney
Lung Antibodies	>24 publications with YBX1 and Lung
Vascular Antibodies	>15 publications with YBX1 and Vascular
Muscle Antibodies	>15 publications with YBX1 and Muscle
Thyroid Antibodies	>13 publications with YBX1 and Thyroid
Mammary Gland Antibodies	>11 publications with YBX1 and Mammary Gland
Embryonic Tissue Antibodies	>11 publications with YBX1 and Embryonic Tissue