Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit for Mouse

Name: Mouse DGAT1 ELISA Kit; Diacylglycerol-O-Acyltransferase Homolog 1; MyBioSource
Brand: MyBioSource
SKU: MBS093985
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS093985

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of DGAT1 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), ELISA Kit

Full Product Name

Mouse Diacylglycerol-O-Acyltransferase Homolog 1 ELISA Kit

Product Gene Name

DGAT1 elisa kit

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples

Detection Range

0.625 ng/ml - 20 ng/mL

Sensitivity

0.1 ng/mL

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

43,831 Da

OMIM

606983

3D Structure

ModBase 3D Structure for Q96PD7

Other Notes

Small volumes of DGAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DGAT1 purchase

MBS093985 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DGAT1. The ELISA analytical biochemical technique of the MBS093985 kit is based on DGAT1 antibody-DGAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DGAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DGAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DGAT1. It may not necessarily be applicable to this product.

NCBI GI #

26024197

NCBI GeneID

84649

NCBI Accession #

NP_115953.2

UniProt Primary Accession #

Q96PD7

UniProt Secondary Accession #

Q5U810; Q68CL3; Q68DJ0; Q8NDB7; Q96BS0; Q9BYE5; A6ND76

UniProt Related Accession #

Q96PD7

GenBank Nucleotide #

NM_032564.4

NCBI Official Full Name

diacylglycerol O-acyltransferase 2 isoform 1

NCBI Official Synonym Full Names

diacylglycerol O-acyltransferase 2

NCBI Official Symbol

DGAT2

NCBI Official Synonym Symbols

ARAT; HMFN1045; GS1999FULL

UniProt Protein Name

Diacylglycerol O-acyltransferase 2

UniProt Synonym Protein Names

Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2

Protein Family

Diacylglycerol O-acyltransferase

UniProt Gene Name

DGAT2

UniProt Synonym Gene Names

ARAT; Retinol O-fatty-acyltransferase

UniProt Entry Name

DGAT2_HUMAN

NCBI Protein Information

diacylglycerol O-acyltransferase 2; diglyceride acyltransferase 2; retinol O-fatty-acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2

NCBI Summary for DGAT1

This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for DGAT1

DGAT2: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Belongs to the diacylglycerol acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.76; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Membrane protein, integral; EC 2.3.1.20; Cofactor and Vitamin Metabolism - retinol; Transferase

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; lipid particle

Molecular Function: 2-acylglycerol O-acyltransferase activity; protein homodimerization activity; retinol O-fatty-acyltransferase activity; diacylglycerol O-acyltransferase activity

Biological Process: sequestering of lipid; cholesterol homeostasis; retinol metabolic process; glycerol metabolic process; phospholipid metabolic process; fatty acid homeostasis; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; diacylglycerol metabolic process

Research Articles on DGAT1

1. Hepatic triacylglycerol synthesis and secretion: DGAT2 as the link between glycaemia and triglyceridaemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DGAT1 elisa kit

Reference Product	PubMed Publications
DGAT1 elisa kit	>98 publications with DGAT1 and DGAT1
PNPLA3 elisa kit	>1 publications with DGAT1 and PNPLA3
PNPLA2 elisa kit	>1 publications with DGAT1 and PNPLA2
LPIN1 elisa kit	>1 publications with DGAT1 and LPIN1

Pathways associated with DGAT1 elisa kit

Products by Pathway	Pathway Diagram
Acyl Chain Remodeling Of DAG And TAG Pathway antibodies	Acyl Chain Remodeling Of DAG And TAG Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerophospholipid Biosynthesis Pathway antibodies	Glycerophospholipid Biosynthesis Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Phospholipid Metabolism Pathway antibodies	Phospholipid Metabolism Pathway Diagram

Diseases associated with DGAT1 elisa kit

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>56 publications with DGAT1 and Fatty Liver
Liver Diseases Antibodies	>36 publications with DGAT1 and Liver Diseases
Hyperinsulinism Antibodies	>19 publications with DGAT1 and Hyperinsulinism
Inflammation Antibodies	>12 publications with DGAT1 and Inflammation
Dyslipidemias Antibodies	>9 publications with DGAT1 and Dyslipidemias
Hypertriglyceridemia Antibodies	>9 publications with DGAT1 and Hypertriglyceridemia
Hyperlipidemias Antibodies	>8 publications with DGAT1 and Hyperlipidemias
Atherosclerosis Antibodies	>8 publications with DGAT1 and Atherosclerosis
Weight Gain Antibodies	>6 publications with DGAT1 and Weight Gain
Non-alcoholic Fatty Liver Disease Antibodies	>5 publications with DGAT1 and Non-alcoholic Fatty Liver Disease

Organs/Tissues associated with DGAT1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>71 publications with DGAT1 and Liver
Blood Antibodies	>44 publications with DGAT1 and Blood
Adipose Tissue Antibodies	>41 publications with DGAT1 and Adipose Tissue
Connective Tissue Antibodies	>24 publications with DGAT1 and Connective Tissue
Muscle Antibodies	>17 publications with DGAT1 and Muscle
Heart Antibodies	>12 publications with DGAT1 and Heart
Intestine Antibodies	>10 publications with DGAT1 and Intestine
Kidney Antibodies	>6 publications with DGAT1 and Kidney
Skin Antibodies	>6 publications with DGAT1 and Skin
Thyroid Antibodies	>4 publications with DGAT1 and Thyroid