Human CYP19A1 ELISA Kit – Cat# MBS940663

Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) ELISA Kit for Human

Name: Human CYP19A1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS940663
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS940663

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP19A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP)… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), ELISA Kit

Full Product Name

Human Cytochrome P450 19A1, CYP19A1 ELISA Kit

Product Gene Name

CYP19A1 elisa kit

Product Synonym Names

Human Cytochrome P450 19A1 (CYP19A1) ELISA kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; cytochrome P450; family 19; subfamily A; polypeptide 1

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

26.7 pg/ml -1000 pg/ml.

Sensitivity

The minimum detectable dose of human CYP19A1 is typically less than 6.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CYP19A1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human CYP19A1. No significant cross-reactivity or interference between human CYP19A1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

57,883 Da

OMIM

613546

3D Structure

ModBase 3D Structure for P11511

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CYP19A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP19A1 purchase

MBS940663 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP19A1. The ELISA analytical biochemical technique of the MBS940663 kit is based on CYP19A1 antibody-CYP19A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP19A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP19A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CYP19A1. It may not necessarily be applicable to this product.

NCBI GI #

13904858

NCBI GeneID

1588

NCBI Accession #

NP_000094.2

UniProt Primary Accession #

P11511

UniProt Secondary Accession #

Q16731; Q3B764; Q58FA0; Q8IYJ7

UniProt Related Accession #

P11511

GenBank Nucleotide #

NM_000103.3

NCBI Official Full Name

aromatase

NCBI Official Synonym Full Names

cytochrome P450, family 19, subfamily A, polypeptide 1

NCBI Official Symbol

CYP19A1

NCBI Official Synonym Symbols

ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

UniProt Protein Name

Aromatase

UniProt Synonym Protein Names

CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase

Protein Family

Aromatase

UniProt Gene Name

CYP19A1

UniProt Synonym Gene Names

ARO1; CYAR; CYP19

UniProt Entry Name

CP19A_HUMAN

NCBI Protein Information

aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)

NCBI Summary for CYP19A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

UniProt Comments for CYP19A1

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.14; Oxidoreductase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; sterol metabolic process; estrogen biosynthetic process; steroid biosynthetic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome

Research Articles on CYP19A1

1. Our results indicate that, in patients with LN-negative, HR-positive breast cancers, genetic polymorphism in CYP19 gene( haplotype AASA) is associated with poor survival of premenopausal women but does not affect survival of postmenopausal women.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYP19A1 elisa kit

Reference Product	PubMed Publications
JUN elisa kit	>26 publications with CYP19A1 and JUN
JUND elisa kit	>2 publications with CYP19A1 and JUND

Pathways associated with CYP19A1 elisa kit

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies	C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies	C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
Cytochrome P450 - Arranged By Substrate Type Pathway antibodies	Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
Endogenous Sterols Pathway antibodies	Endogenous Sterols Pathway Diagram
Estrogen Biosynthesis Pathway antibodies	Estrogen Biosynthesis Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Steroid Hormones And Vitamins A And D Pathway antibodies	Metabolism Of Steroid Hormones And Vitamins A And D Pathway Diagram
Ovarian Infertility Genes Pathway antibodies	Ovarian Infertility Genes Pathway Diagram

Diseases associated with CYP19A1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>4342 publications with CYP19A1 and Neoplasms
Breast Neoplasms Antibodies	>3506 publications with CYP19A1 and Breast Neoplasms
Drug Toxicity Antibodies	>591 publications with CYP19A1 and Drug Toxicity
Ovarian Neoplasms Antibodies	>378 publications with CYP19A1 and Ovarian Neoplasms
Ovarian Diseases Antibodies	>377 publications with CYP19A1 and Ovarian Diseases
Neoplasms, Experimental Antibodies	>343 publications with CYP19A1 and Neoplasms, Experimental
Adenocarcinoma Antibodies	>343 publications with CYP19A1 and Adenocarcinoma
Uterine Neoplasms Antibodies	>316 publications with CYP19A1 and Uterine Neoplasms
Endometrial Neoplasms Antibodies	>309 publications with CYP19A1 and Endometrial Neoplasms
Infertility, Female Antibodies	>284 publications with CYP19A1 and Infertility, Female

Organs/Tissues associated with CYP19A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>2080 publications with CYP19A1 and Ovary
Blood Antibodies	>1807 publications with CYP19A1 and Blood
Brain Antibodies	>1460 publications with CYP19A1 and Brain
Testis Antibodies	>868 publications with CYP19A1 and Testis
Placenta Antibodies	>782 publications with CYP19A1 and Placenta
Liver Antibodies	>422 publications with CYP19A1 and Liver
Embryonic Tissue Antibodies	>293 publications with CYP19A1 and Embryonic Tissue
Vascular Antibodies	>195 publications with CYP19A1 and Vascular
Adrenal Gland Antibodies	>179 publications with CYP19A1 and Adrenal Gland
Lung Antibodies	>117 publications with CYP19A1 and Lung