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Phospholipase A2, Group V (PLA2G5) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS941705

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G5 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
phospholipase A2, group V (PLA2G5), ELISA Kit
Full Product Name
Mouse Calcium-dependent phospholipase A2, PLA2G5 ELISA Kit
Product Gene Name
PLA2G5 elisa kit
Product Synonym Names
Mouse Calcium-dependent phospholipase A2 (PLA2G5) ELISA kit; DKFZp686C2294; GV-PLA2; MGC46205; PLA2-10; hVPLA (2); Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group V
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G5. No significant cross-reactivity or interference between Mouse PLA2G5 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Molecular Weight
15,859 Da
3D Structure
ModBase 3D Structure for P97391
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Lipogenesis and lipometabolism
Protein Biological Process 3
Lipid degradation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of PLA2G5 elisa kit

Mouse PLA2G5 ELISA Kit – Cat# MBS941705

Other Notes

Small volumes of PLA2G5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLA2G5 purchase

MBS941705 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phospholipase A2, group V (PLA2G5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLA2G5. The ELISA analytical biochemical technique of the MBS941705 kit is based on PLA2G5 antibody-PLA2G5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLA2G5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLA2G5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLA2G5. It may not necessarily be applicable to this product.

NCBI GI #
171906563
NCBI GeneID
18784
NCBI Accession #
NP_001116426.1
UniProt Primary Accession #
P97391
UniProt Secondary Accession #
Q9QZU6
UniProt Related Accession #
P97391
GenBank Nucleotide #
NM_001122954.1
NCBI Official Full Name
calcium-dependent phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group V
NCBI Official Symbol
Pla2g5
NCBI Official Synonym Symbols
PLA2; sPLA2
UniProt Protein Name
Calcium-dependent phospholipase A2
UniProt Synonym Protein Names
Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5
Protein Family
Calcium-dependent phospholipase
UniProt Gene Name
Pla2g5
UniProt Entry Name
PA2G5_MOUSE
NCBI Protein Information
calcium-dependent phospholipase A2; PLA2-10; group V phospholipase A2; phosphatidylcholine 2-acylhydrolase 5

UniProt Comments for PLA2G5

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family.

Protein type: Phospholipase; Cell surface; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid

Cellular Component: Golgi apparatus; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane

Molecular Function: heparin binding; phospholipase A2 activity; hydrolase activity; calcium-dependent phospholipase A2 activity; metal ion binding; calcium ion binding; receptor binding

Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; phospholipid metabolic process; lipid metabolic process; arachidonic acid secretion; lipid catabolic process
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Research Articles on PLA2G5

1. Key Data shows the role of group v secreted phospholipase A2 in Th2 cytokine and dendritic cell-driven airway hyperresponsiveness and remodeling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PLA2G5 elisa kit

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram

Diseases associated with PLA2G5 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >14 publications with PLA2G5 and Inflammation
 Cardiovascular Diseases Antibodies  >8 publications with PLA2G5 and Cardiovascular Diseases
 Neoplasms Antibodies  >8 publications with PLA2G5 and Neoplasms
 Arteriosclerosis Antibodies  >6 publications with PLA2G5 and Arteriosclerosis
 Atherosclerosis Antibodies  >6 publications with PLA2G5 and Atherosclerosis
 Necrosis Antibodies  >4 publications with PLA2G5 and Necrosis
 Carcinoma Antibodies  >3 publications with PLA2G5 and Carcinoma
 Lung Diseases Antibodies  >3 publications with PLA2G5 and Lung Diseases
 Myocardial Ischemia Antibodies  >3 publications with PLA2G5 and Myocardial Ischemia
 Fatty Liver Antibodies  >3 publications with PLA2G5 and Fatty Liver

Organs/Tissues associated with PLA2G5 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with PLA2G5 and Blood
 Heart Antibodies  >5 publications with PLA2G5 and Heart
 Kidney Antibodies  >5 publications with PLA2G5 and Kidney
 Lung Antibodies  >4 publications with PLA2G5 and Lung
 Intestine Antibodies  >3 publications with PLA2G5 and Intestine
 Eye Antibodies  >3 publications with PLA2G5 and Eye
 Spleen Antibodies  >1 publications with PLA2G5 and Spleen
 Brain Antibodies  >1 publications with PLA2G5 and Brain
 Stomach Antibodies  >1 publications with PLA2G5 and Stomach
 Joint Antibodies  >1 publications with PLA2G5 and Joint
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