MASP2 (Mannose-Associated Serine Protease 2) ELISA Kit for Human

Name: Human MASP2 ELISA Kit; Mannose Associated Serine Protease 2; MyBioSource
Brand: MyBioSource
SKU: MBS094319
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS094319

This human MASP2 (Mannose-Associated Serine Protease 2) ELISA kit uses a sandwich immunoassay format to quantify MASP2 in serum, plasma, and tissue homogenates, supporting research into lectin complement pathway activation and innate immune signaling.… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Mannose Associated Serine Protease 2 (MASP2), ELISA Kit

Full Product Name

Human Mannose Associated Serine Protease 2 ELISA Kit

Product Gene Name

MASP2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Undiluted original Human serum, plasma or tissue homogenates samples

Detection Range

62.5pg/ml-2000pg/ml

Sensitivity

10pg/ml

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

75,702 Da

OMIM

605102

3D Structure

ModBase 3D Structure for O00187

Other Notes

Small volumes of MASP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MASP2 purchase

MBS094319 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mannose Associated Serine Protease 2 (MASP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MASP2. The ELISA analytical biochemical technique of the MBS094319 kit is based on MASP2 antibody-MASP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MASP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MASP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MASP2. It may not necessarily be applicable to this product.

NCBI GI #

21264363

NCBI GeneID

10747

NCBI Accession #

NP_006601.2

UniProt Primary Accession #

O00187

UniProt Secondary Accession #

O75754; Q5TEQ5; Q5TER0; Q96QG4; Q9BZH0; Q9H498; Q9H499; Q9UBP3; Q9UC48; A8K458; A8MWJ2

UniProt Related Accession #

O00187

GenBank Nucleotide #

NM_006610.3

NCBI Official Full Name

mannan-binding lectin serine protease 2 isoform 1 preproprotein

NCBI Official Synonym Full Names

mannan-binding lectin serine peptidase 2

NCBI Official Symbol

MASP2

NCBI Official Synonym Symbols

sMAP; MAP19; MASP-2; MASP1P1

UniProt Protein Name

Mannan-binding lectin serine protease 2

UniProt Synonym Protein Names

MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2

Protein Family

Mannan-binding lectin serine protease

UniProt Gene Name

MASP2

UniProt Synonym Gene Names

MASP-2

UniProt Entry Name

MASP2_HUMAN

NCBI Protein Information

mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1

NCBI Summary for MASP2

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

UniProt Comments for MASP2

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.104; Secreted

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding

Biological Process: innate immune response; proteolysis; complement activation, lectin pathway; complement activation, classical pathway; complement activation

Disease: Masp2 Deficiency

Research Articles on MASP2

1. Data indicate that MASP-1 and MASP-2 can readily form heterodimers after dissociation and re-association, however, in the presence of Ca(2+) exchange of subunits is slow between the homodimers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MASP2 elisa kit

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement Cascade Pathway antibodies	Complement Cascade Pathway Diagram
Creation Of C4 And C2 Activators Pathway antibodies	Creation Of C4 And C2 Activators Pathway Diagram
Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway antibodies	Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Initial Triggering Of Complement Pathway antibodies	Initial Triggering Of Complement Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Lectin Pathway Of Complement Activation antibodies	Lectin Pathway Of Complement Activation Diagram

Diseases associated with MASP2 elisa kit

Disease Name	Pubmed Publications
MASP2 Deficiency Antibodies	>50 publications with MASP2 and MASP2 Deficiency
Inflammation Antibodies	>28 publications with MASP2 and Inflammation
Nervous System Diseases Antibodies	>7 publications with MASP2 and Nervous System Diseases
Liver Diseases Antibodies	>7 publications with MASP2 and Liver Diseases
Kidney Diseases Antibodies	>6 publications with MASP2 and Kidney Diseases
Hepatitis Antibodies	>5 publications with MASP2 and Hepatitis
Fibrosis Antibodies	>5 publications with MASP2 and Fibrosis
Disease Models, Animal Antibodies	>4 publications with MASP2 and Disease Models, Animal
Liver Neoplasms Antibodies	>3 publications with MASP2 and Liver Neoplasms
Nephritis Antibodies	>3 publications with MASP2 and Nephritis

Organs/Tissues associated with MASP2 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>22 publications with MASP2 and Liver
Lung Antibodies	>7 publications with MASP2 and Lung
Brain Antibodies	>6 publications with MASP2 and Brain
Placenta Antibodies	>1 publications with MASP2 and Placenta
Thyroid Antibodies	>1 publications with MASP2 and Thyroid
Embryonic Tissue Antibodies	>1 publications with MASP2 and Embryonic Tissue