Sulfatase 1 (SULF1) ELISA Kit for Human

Name: Human SULF1 ELISA Kit; Sulfatase 1; MyBioSource
Brand: MyBioSource
SKU: MBS943783
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS943783

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SULF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SULF1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

sulfatase 1 (SULF1), ELISA Kit

Full Product Name

Human Extracellular sulfatase Sulf-1, SULF1 ELISA Kit

Product Gene Name

SULF1 elisa kit

Product Synonym Names

Human Extracellular sulfatase Sulf-1 (SULF1) ELISA kit; FLJ30905; FLJ38022; FLJ41750; HSULF-1; KIAA1077; SULF-1; sulfatase FP; sulfatase 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

7.8 pg/ml-500 pg/ml

Sensitivity

< 1.95 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human SULF1. No significant cross-reactivity or interference between human SULF1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

101,027 Da

OMIM

610012

3D Structure

ModBase 3D Structure for Q8IWU6

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SULF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SULF1 purchase

MBS943783 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sulfatase 1 (SULF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SULF1. The ELISA analytical biochemical technique of the MBS943783 kit is based on SULF1 antibody-SULF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SULF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SULF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SULF1. It may not necessarily be applicable to this product.

NCBI GI #

189571638

NCBI GeneID

23213

NCBI Accession #

NP_001121676.1

UniProt Primary Accession #

Q8IWU6

UniProt Secondary Accession #

Q86YV8; Q8NCA2; Q9UPS5

UniProt Related Accession #

Q8IWU6

GenBank Nucleotide #

NM_001128204.1

NCBI Official Full Name

extracellular sulfatase Sulf-1

NCBI Official Synonym Full Names

sulfatase 1

NCBI Official Symbol

SULF1

NCBI Official Synonym Symbols

SULF-1; HSULF-1

UniProt Protein Name

Extracellular sulfatase Sulf-1

Protein Family

Extracellular sulfatase

UniProt Gene Name

SULF1

UniProt Synonym Gene Names

KIAA1077; hSulf-1

UniProt Entry Name

SULF1_HUMAN

NCBI Protein Information

extracellular sulfatase Sulf-1; sulfatase FP

NCBI Summary for SULF1

This gene encodes an extracellular heparan sulfate endosulfatase. The encoded enzyme selectively removes 6-O-sulfate groups from heparan sulfate chains of heparan sulfate proteoglycans (HSPGs). The enzyme is secreted through the Golgi and is subsequently localized to the cell surface. The expression of this gene may be down-regulated in several types of cancer, including hepatocellular (HCC), ovarian and breast cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

UniProt Comments for SULF1

SULF1: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation. Belongs to the sulfatase family.

Protein type: Hydrolase; EC 3.1.6.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q13.2

Cellular Component: Golgi apparatus; extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane; lipid raft

Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; apoptosis; embryonic skeletal development; glomerular basement membrane development; glomerular filtration; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; negative regulation of angiogenesis; cartilage development; esophagus smooth muscle contraction; negative regulation of endothelial cell proliferation; heparan sulfate proteoglycan metabolic process; kidney development; vascular endothelial growth factor receptor signaling pathway; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; positive regulation of BMP signaling pathway

Research Articles on SULF1

1. identification of markers including SULF1 may improve detection of this disease at its earliest stages improving patient treatment and prognosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with SULF1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>38 publications with SULF1 and Neoplasms
Carcinoma Antibodies	>22 publications with SULF1 and Carcinoma
Adenocarcinoma Antibodies	>13 publications with SULF1 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>11 publications with SULF1 and Carcinoma, Hepatocellular
Ovarian Neoplasms Antibodies	>11 publications with SULF1 and Ovarian Neoplasms
Skin Diseases Antibodies	>9 publications with SULF1 and Skin Diseases
Breast Neoplasms Antibodies	>9 publications with SULF1 and Breast Neoplasms
Liver Diseases Antibodies	>8 publications with SULF1 and Liver Diseases
Neoplasms, Experimental Antibodies	>7 publications with SULF1 and Neoplasms, Experimental
Neoplasm Invasiveness Antibodies	>7 publications with SULF1 and Neoplasm Invasiveness

Organs/Tissues associated with SULF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>15 publications with SULF1 and Embryonic Tissue
Brain Antibodies	>10 publications with SULF1 and Brain
Bone Antibodies	>10 publications with SULF1 and Bone
Vascular Antibodies	>10 publications with SULF1 and Vascular
Muscle Antibodies	>8 publications with SULF1 and Muscle
Lung Antibodies	>7 publications with SULF1 and Lung
Kidney Antibodies	>5 publications with SULF1 and Kidney
Heart Antibodies	>5 publications with SULF1 and Heart
Nerve Antibodies	>3 publications with SULF1 and Nerve
Skin Antibodies	>3 publications with SULF1 and Skin