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Wingless-Type MMTV Integration Site Family, Member 3a (WNT3A) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS943878

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3A present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
wingless-type MMTV integration site family, member 3A (WNT3A), ELISA Kit
Full Product Name
Mouse Protein Wnt-3a, WNT3A ELISA Kit
Product Gene Name
WNT3A elisa kit
Product Synonym Names
Mouse Protein Wnt-3a (WNT3A) ELISA kit; MGC119418; MGC119419; MGC119420; ; wingless-type MMTV integration site family; member 3A
Species Reactivity
Mouse
Assay Type
Sandwich (Quantitative)
Samples
Serum, plasma, tissue homogenates.
Detection Range
23.5 pg/ml-1500 pg/ml.
Sensitivity
5.8 pg/ml.
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse WNT3A. No significant cross-reactivity or interference between mouse WNT3A and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
39,258 Da
3D Structure
ModBase 3D Structure for P27467
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of WNT3A elisa kit

Mouse WNT3A ELISA Kit – Cat# MBS943878

Other Notes

Small volumes of WNT3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT3A purchase

MBS943878 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the wingless-type MMTV integration site family, member 3A (WNT3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT3A. The ELISA analytical biochemical technique of the MBS943878 kit is based on WNT3A antibody-WNT3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). The Wnt gene signaling pathway contains at least 14 known human and mouse homologous MMTV integration site family member wingless type protein (s). Human mouse rat Wnt 3a have similar physiological roles and show similar cell expression patterns and quantitative levels by ELISA. The MyBiosource wingless type MMTV family member 3a ELISA kit is a sandwich format with rebound antibodies, with very low LLD - below 5.8 pg/ml is typical - and a high degree of intra assay and inter assay precision and sensitive biotin-streptavidin enzymatic detection, making this and human or other specific Wnt3a gene products ELISA kits an excellent technical choice. For exact details of the use of the kit 1 plate design, refer to the included technical tech manual with PubMed articles for further suggestions.

Additional Details

NCBI/Uniprot data below describe general gene information for WNT3A. It may not necessarily be applicable to this product.

NCBI GI #
7106447
NCBI GeneID
22416
NCBI Accession #
NP_033548.1
UniProt Primary Accession #
P27467
UniProt Related Accession #
P27467
GenBank Nucleotide #
NM_009522.2
NCBI Official Full Name
protein Wnt-3a
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol
Wnt3a
NCBI Official Synonym Symbols
vt; Wnt-3a
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
Wnt3a
UniProt Synonym Gene Names
Wnt-3a
UniProt Entry Name
WNT3A_MOUSE
NCBI Protein Information
protein Wnt-3a; vestigial tail; wingless-related MMTV integration site 3A

UniProt Comments for WNT3A

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region

Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; receptor binding; frizzled-2 binding

Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell proliferation in forebrain; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; palate development; regulation of axonogenesis; signal transduction; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; BMP signaling pathway; anterior/posterior pattern formation; mammary gland development; cell-cell signaling; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of B cell proliferation; somatic stem cell division; mesoderm development; hemopoiesis; heart looping; dorsoventral neural tube patterning; positive regulation of cytokine production; platelet activation; somitogenesis; inner ear morphogenesis; Wnt receptor signaling pathway; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; organ morphogenesis; axonogenesis; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry
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Research Articles on WNT3A

1. Wnt3a/beta-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT3A elisa kit

 Reference Product  PubMed Publications
 Porcn elisa kit  >4 publications with WNT3A and Porcn

Pathways associated with WNT3A elisa kit

 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 ESC Pluripotency Pathways antibodies  ESC Pluripotency Pathways Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram

Diseases associated with WNT3A elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >118 publications with WNT3A and Neoplasms
 Gastrointestinal Diseases Antibodies  >30 publications with WNT3A and Gastrointestinal Diseases
 Skin Diseases Antibodies  >23 publications with WNT3A and Skin Diseases
 Congenital Abnormalities Antibodies  >18 publications with WNT3A and Congenital Abnormalities
 Nervous System Diseases Antibodies  >17 publications with WNT3A and Nervous System Diseases
 Necrosis Antibodies  >17 publications with WNT3A and Necrosis
 Breast Neoplasms Antibodies  >16 publications with WNT3A and Breast Neoplasms
 Fibrosis Antibodies  >15 publications with WNT3A and Fibrosis
 Hematologic Diseases Antibodies  >12 publications with WNT3A and Hematologic Diseases
 Liver Diseases Antibodies  >11 publications with WNT3A and Liver Diseases

Organs/Tissues associated with WNT3A elisa kit

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >112 publications with WNT3A and Embryonic Tissue
 Lung Antibodies  >22 publications with WNT3A and Lung
 Testis Antibodies  >5 publications with WNT3A and Testis
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