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DNA-Damage-Inducible Transcript 4 (DDIT4) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS944137

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DDIT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDIT4 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA-damage-inducible transcript 4 (DDIT4), ELISA Kit
Full Product Name
Human DNA damage-inducible transcript 4 protein, DDIT4 ELISA Kit
Product Gene Name
DDIT4 elisa kit
Product Synonym Names
Human DNA damage-inducible transcript 4 protein (DDIT4) ELISA kit; Dig2; FLJ20500; REDD-1; REDD1; RP11-442H21.1; RTP801; HIF-1 responsive RTP801 (RTP801) ; RTP801; DNA-damage-inducible transcript 4
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (CSF)
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
< 0.039 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of human DDIT4. No significant cross-reactivity or interference between human DDIT4 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
25,371 Da
OMIM
607729
3D Structure
ModBase 3D Structure for Q9NX09
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of DDIT4 elisa kit

Human DDIT4 ELISA Kit – Cat# MBS944137

Other Notes

Small volumes of DDIT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDIT4 purchase

MBS944137 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA-damage-inducible transcript 4 (DDIT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDIT4. The ELISA analytical biochemical technique of the MBS944137 kit is based on DDIT4 antibody-DDIT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDIT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDIT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DDIT4. It may not necessarily be applicable to this product.

NCBI GI #
9506687
NCBI GeneID
54541
NCBI Accession #
NP_061931.1
UniProt Primary Accession #
Q9NX09
UniProt Secondary Accession #
Q9H0S3
UniProt Related Accession #
Q9NX09
GenBank Nucleotide #
NM_019058.2
NCBI Official Full Name
DNA damage-inducible transcript 4 protein
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 4
NCBI Official Symbol
DDIT4
NCBI Official Synonym Symbols
Dig2; REDD1; REDD-1
UniProt Protein Name
DNA damage-inducible transcript 4 protein
UniProt Synonym Protein Names
HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
Protein Family
DNA damage-inducible transcript 4 protein
UniProt Gene Name
DDIT4
UniProt Synonym Gene Names
REDD1; RTP801; REDD-1
UniProt Entry Name
DDIT4_HUMAN
NCBI Protein Information
DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1

UniProt Comments for DDIT4

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol

Biological Process: neuron differentiation; cell proliferation; protein complex disassembly; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; response to hypoxia; neuron migration; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus
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Research Articles on DDIT4

1. these postmortem and preclinical findings identify REDD1 as a critical mediator of the atrophy of neurons and depressive behavior caused by chronic stress exposure

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DDIT4 elisa kit

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with DDIT4 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >25 publications with DDIT4 and Neoplasms
 Carcinoma Antibodies  >11 publications with DDIT4 and Carcinoma
 Nervous System Diseases Antibodies  >9 publications with DDIT4 and Nervous System Diseases
 Drug Toxicity Antibodies  >8 publications with DDIT4 and Drug Toxicity
 Brain Diseases Antibodies  >7 publications with DDIT4 and Brain Diseases
 Breast Neoplasms Antibodies  >6 publications with DDIT4 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with DDIT4 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >4 publications with DDIT4 and Adenocarcinoma
 Leukemia Antibodies  >4 publications with DDIT4 and Leukemia
 Carcinoma, Hepatocellular Antibodies  >3 publications with DDIT4 and Carcinoma, Hepatocellular

Organs/Tissues associated with DDIT4 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >10 publications with DDIT4 and Muscle
 Blood Antibodies  >7 publications with DDIT4 and Blood
 Brain Antibodies  >7 publications with DDIT4 and Brain
 Liver Antibodies  >6 publications with DDIT4 and Liver
 Prostate Antibodies  >4 publications with DDIT4 and Prostate
 Lung Antibodies  >3 publications with DDIT4 and Lung
 Nerve Antibodies  >2 publications with DDIT4 and Nerve
 Kidney Antibodies  >2 publications with DDIT4 and Kidney
 Ovary Antibodies  >1 publications with DDIT4 and Ovary
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