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Annexin A1 (ANXA1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS944251

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANXA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANXA1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
annexin A1 (ANXA1), ELISA Kit
Full Product Name
Mouse Annexin A1, ANXA1 ELISA Kit
Product Gene Name
ANXA1 elisa kit
Product Synonym Names
Mouse Annexin A1 (ANXA1) ELISA kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; annexin A1
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
15.6 ng/ml-1000 ng/ml
Sensitivity
< 3.9 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ANXA1. No significant cross-reactivity or interference between mouse ANXA1 and analogues was observed.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
38,734 Da
3D Structure
ModBase 3D Structure for P10107
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ANXA1 elisa kit

Mouse ANXA1 ELISA Kit – Cat# MBS944251

Other Notes

Small volumes of ANXA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANXA1 purchase

MBS944251 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the annexin A1 (ANXA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANXA1. The ELISA analytical biochemical technique of the MBS944251 kit is based on ANXA1 antibody-ANXA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANXA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANXA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.

NCBI GI #
124517663
NCBI GeneID
16952
NCBI Accession #
NP_034860.2
UniProt Primary Accession #
P10107
UniProt Related Accession #
P10107
GenBank Nucleotide #
NM_010730.2
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
Anxa1
NCBI Official Synonym Symbols
Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Protein Family
Annexin
UniProt Gene Name
Anxa1
UniProt Synonym Gene Names
Anx1; Lpc-1; Lpc1
UniProt Entry Name
ANXA1_MOUSE
NCBI Protein Information
annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin 1; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein

UniProt Comments for ANXA1

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Cellular Component: extracellular space; focal adhesion; protein complex; mast cell granule; cell surface; cornified envelope; cilium; cell projection; membrane; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; phospholipase inhibitor activity; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; calcium-dependent protein binding; helicase activity; single-stranded DNA binding

Biological Process: negative regulation of protein secretion; positive regulation of neutrophil apoptosis; positive regulation of apoptosis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; neutrophil homeostasis; signal transduction; arachidonic acid secretion; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; positive regulation of prostaglandin biosynthetic process
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Research Articles on ANXA1

1. Data show an increase of extracellular Annexin A1 (ANXA1) starting from the initial phases of skeletal muscle cell differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ANXA1 elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Prostaglandin Synthesis And Regulation Pathway antibodies  Prostaglandin Synthesis And Regulation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with ANXA1 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >73 publications with ANXA1 and Neoplasms
 Inflammation Antibodies  >44 publications with ANXA1 and Inflammation
 Carcinoma Antibodies  >42 publications with ANXA1 and Carcinoma
 Nervous System Diseases Antibodies  >17 publications with ANXA1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >14 publications with ANXA1 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >14 publications with ANXA1 and Breast Neoplasms
 Gastrointestinal Diseases Antibodies  >12 publications with ANXA1 and Gastrointestinal Diseases
 Lung Diseases Antibodies  >10 publications with ANXA1 and Lung Diseases
 Necrosis Antibodies  >9 publications with ANXA1 and Necrosis
 Brain Diseases Antibodies  >9 publications with ANXA1 and Brain Diseases

Organs/Tissues associated with ANXA1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >57 publications with ANXA1 and Blood
 Brain Antibodies  >18 publications with ANXA1 and Brain
 Lung Antibodies  >15 publications with ANXA1 and Lung
 Pituitary Gland Antibodies  >12 publications with ANXA1 and Pituitary Gland
 Liver Antibodies  >8 publications with ANXA1 and Liver
 Muscle Antibodies  >7 publications with ANXA1 and Muscle
 Bone Antibodies  >7 publications with ANXA1 and Bone
 Kidney Antibodies  >5 publications with ANXA1 and Kidney
 Stomach Antibodies  >5 publications with ANXA1 and Stomach
 Bone Marrow Antibodies  >5 publications with ANXA1 and Bone Marrow
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