Fc Fragment of IgG, Low Affinity Iiia, Receptor (Cd16a) (FCGR3A) ELISA Kit for Human

Name: Human FCGR3A ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS944475
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS944475

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FBXO32 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FBXO32 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), ELISA Kit

Full Product Name

Human Low affinity immunoglobulin gamma Fc region receptor III-A, FCGR3A ELISA Kit

Product Gene Name

FCGR3A elisa kit

Product Synonym Names

Human Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A) ELISA kit; RP11-5K23.1; CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; Fc fragment of IgG; low affinity III; receptor for (CD16) ; Fc gamma receptor III-A; Fc-gamma RIII-alpha; Fc-gamma RIIIa; F; Fc fragment of IgG; low affinity IIIa; receptor (CD16a)

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

7.8 pg/ml-500 pg/ml

Sensitivity

1.95 pg/ml.

Specificity

This assay has high sensitivity and excellent specificity for detection of human FBXO32. No significant cross-reactivity or interference between human FBXO32 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess

Molecular Weight

29,089 Da

OMIM

146740

3D Structure

ModBase 3D Structure for P08637

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FCGR3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCGR3A purchase

MBS944475 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FCGR3A. The ELISA analytical biochemical technique of the MBS944475 kit is based on FCGR3A antibody-FCGR3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FCGR3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FCGR3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR3A. It may not necessarily be applicable to this product.

NCBI GI #

50726979

NCBI GeneID

2214

NCBI Accession #

NP_000560.5

UniProt Primary Accession #

P08637

UniProt Secondary Accession #

Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9

UniProt Related Accession #

P08637

GenBank Nucleotide #

NM_000569.6

NCBI Official Full Name

low affinity immunoglobulin gamma Fc region receptor III-A isoform a

NCBI Official Synonym Full Names

Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

NCBI Official Symbol

FCGR3A

NCBI Official Synonym Symbols

CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA

UniProt Protein Name

Low affinity immunoglobulin gamma Fc region receptor III-A

UniProt Synonym Protein Names

CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: CD16a

Protein Family

Low affinity immunoglobulin gamma Fc region receptor

UniProt Gene Name

FCGR3A

UniProt Synonym Gene Names

CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

UniProt Entry Name

FCG3A_HUMAN

NCBI Protein Information

low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16)

NCBI Summary for FCGR3A

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for FCGR3A

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20

Research Articles on FCGR3A

1. Describe biosensing techniques to evaluate the glycosylation pattern of monoclonal antibodies using the extracellular domains of CD16a.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FCGR3A elisa kit

Reference Product	PubMed Publications
FCGR1A elisa kit	>6 publications with FCGR3A and FCGR1A
CD4 elisa kit	>4 publications with FCGR3A and CD4
PTPRC elisa kit	>1 publications with FCGR3A and PTPRC

Pathways associated with FCGR3A elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Leishmaniasis Pathway antibodies	Leishmaniasis Pathway Diagram
Leishmaniasis Pathway antibodies	Leishmaniasis Pathway Diagram
Natural Killer Cell Mediated Cytotoxicity Pathway antibodies	Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
Natural Killer Cell Mediated Cytotoxicity Pathway antibodies	Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram

Diseases associated with FCGR3A elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>75 publications with FCGR3A and Neoplasms
Lymphoma Antibodies	>39 publications with FCGR3A and Lymphoma
Arthritis, Rheumatoid Antibodies	>30 publications with FCGR3A and Arthritis, Rheumatoid
Lupus Erythematosus, Systemic Antibodies	>20 publications with FCGR3A and Lupus Erythematosus, Systemic
Intestinal Diseases Antibodies	>19 publications with FCGR3A and Intestinal Diseases
Necrosis Antibodies	>19 publications with FCGR3A and Necrosis
Hematologic Diseases Antibodies	>17 publications with FCGR3A and Hematologic Diseases
Drug Toxicity Antibodies	>11 publications with FCGR3A and Drug Toxicity
Kidney Diseases Antibodies	>8 publications with FCGR3A and Kidney Diseases
Leukemia Antibodies	>8 publications with FCGR3A and Leukemia

Organs/Tissues associated with FCGR3A elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>106 publications with FCGR3A and Blood
Ovary Antibodies	>6 publications with FCGR3A and Ovary
Kidney Antibodies	>5 publications with FCGR3A and Kidney
Lung Antibodies	>3 publications with FCGR3A and Lung
Liver Antibodies	>2 publications with FCGR3A and Liver
Brain Antibodies	>1 publications with FCGR3A and Brain