Hemoglobin, Delta (HBD) ELISA Kit for Human

Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HBD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HBD. A competitive inhibition reaction is launched between HBD (Standards or samples) and biotin-conjugated HBD with the pre-coated HBD antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of HBD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of HBD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS944502 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hemoglobin, delta (HBD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HBD. The ELISA analytical biochemical technique of the MBS944502 kit is based on HBD antibody-HBD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HBD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HBD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).