Phenylalanine Hydroxylase (PAH) ELISA Kit for Mouse

Name: Mouse PAH ELISA Kit; Phenylalanine Hydroxylase; MyBioSource
Brand: MyBioSource
SKU: MBS946253
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS946253

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAH present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

phenylalanine hydroxylase (PAH), ELISA Kit

Full Product Name

Mouse Phenylalanine-4-hydroxylase, PAH ELISA Kit

Product Gene Name

PAH elisa kit

Product Synonym Names

Mouse Phenylalanine-4-hydroxylase (PAH) ELISA kit; PH; PKU; PKU1; ; phenylalanine hydroxylase

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

23.5 pg/ml -1500 pg/ml.

Sensitivity

The minimum detectable dose of mouse PAH is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse PAH. No significant cross-reactivity or interference between mouse PAH and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

51,900 Da

3D Structure

ModBase 3D Structure for P16331

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAH purchase

MBS946253 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phenylalanine hydroxylase (PAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAH. The ELISA analytical biochemical technique of the MBS946253 kit is based on PAH antibody-PAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PAH. It may not necessarily be applicable to this product.

NCBI GI #

171543886

NCBI GeneID

18478

NCBI Accession #

NP_032803.2

UniProt Primary Accession #

P16331

UniProt Secondary Accession #

Q91WV1

UniProt Related Accession #

P16331

GenBank Nucleotide #

NM_008777.3

NCBI Official Full Name

phenylalanine-4-hydroxylase

NCBI Official Synonym Full Names

phenylalanine hydroxylase

NCBI Official Symbol

Pah

NCBI Official Synonym Symbols

AW106920

UniProt Protein Name

Phenylalanine-4-hydroxylase

UniProt Synonym Protein Names

Phe-4-monooxygenase

Protein Family

Phenylalanine-4-hydroxylase

UniProt Gene Name

Pah

UniProt Synonym Gene Names

PAH

UniProt Entry Name

PH4H_MOUSE

NCBI Protein Information

phenylalanine-4-hydroxylase; phe-4-monooxygenase

UniProt Comments for PAH

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Oxidoreductase; EC 1.14.16.1

Molecular Function: amino acid binding; protein homodimerization activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; iron ion binding; metal ion binding; phenylalanine 4-monooxygenase activity; oxidoreductase activity; cofactor binding; monooxygenase activity; catalytic activity

Biological Process: L-phenylalanine metabolic process; tyrosine biosynthetic process; L-phenylalanine catabolic process; tetrahydrobiopterin metabolic process; aromatic amino acid family metabolic process; metabolic process; pteridine and derivative metabolic process; protein amino acid hydroxylation

Research Articles on PAH

1. A single injection of PAH-RBCs was able to decrease Phe levels by nearly 80% in normal mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PAH elisa kit

Products by Pathway	Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Phenylalanine And Tyrosine Catabolism Pathway antibodies	Phenylalanine And Tyrosine Catabolism Pathway Diagram
Phenylalanine Metabolism Pathway antibodies	Phenylalanine Metabolism Pathway Diagram
Phenylalanine Metabolism Pathway antibodies	Phenylalanine Metabolism Pathway Diagram
Phenylalanine, Tyrosine And Tryptophan Biosynthesis Pathway antibodies	Phenylalanine, Tyrosine And Tryptophan Biosynthesis Pathway Diagram
Phenylalanine, Tyrosine And Tryptophan Biosynthesis Pathway antibodies	Phenylalanine, Tyrosine And Tryptophan Biosynthesis Pathway Diagram
Phenylalanine Degradation I (aerobic) Pathway antibodies	Phenylalanine Degradation I (aerobic) Pathway Diagram
Tyrosine Biosynthesis II Pathway antibodies	Tyrosine Biosynthesis II Pathway Diagram

Diseases associated with PAH elisa kit

Disease Name	Pubmed Publications
Hypertension Antibodies	>2303 publications with PAH and Hypertension
Drug Toxicity Antibodies	>1232 publications with PAH and Drug Toxicity
Neoplasms Antibodies	>682 publications with PAH and Neoplasms
Nervous System Diseases Antibodies	>557 publications with PAH and Nervous System Diseases
Brain Diseases Antibodies	>506 publications with PAH and Brain Diseases
Brain Diseases, Metabolic Antibodies	>467 publications with PAH and Brain Diseases, Metabolic
Phenylketonurias Antibodies	>462 publications with PAH and Phenylketonurias
Heart Failure Antibodies	>359 publications with PAH and Heart Failure
Kidney Diseases Antibodies	>343 publications with PAH and Kidney Diseases
Disease Models, Animal Antibodies	>295 publications with PAH and Disease Models, Animal

Organs/Tissues associated with PAH elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2210 publications with PAH and Blood
Kidney Antibodies	>1540 publications with PAH and Kidney
Lung Antibodies	>1118 publications with PAH and Lung
Liver Antibodies	>847 publications with PAH and Liver
Muscle Antibodies	>426 publications with PAH and Muscle
Brain Antibodies	>229 publications with PAH and Brain
Embryonic Tissue Antibodies	>52 publications with PAH and Embryonic Tissue
Stomach Antibodies	>40 publications with PAH and Stomach
Pancreas Antibodies	>11 publications with PAH and Pancreas
Salivary Gland Antibodies	>1 publications with PAH and Salivary Gland