Human WFIKKN2 ELISA Kit – Cat# MBS946744

WAP, Follistatin/Kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2) ELISA Kit for Human

Name: Human WFIKKN2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS946744
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS946744

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WFIKKN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WFIKKN2 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 (WFIKKN2), ELISA Kit

Full Product Name

Human WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2, WFIKKN2 ELISA Kit

Product Gene Name

WFIKKN2 elisa kit

Product Synonym Names

Human WAP; kazal; immunoglobulin; kunitz and NTR domain-containing protein 2 (WFIKKN2) ELISA kit; WFIKKNRP; WAP; FS; Ig; two KU and NTR module related protein; WFIKKN-related protein; WFIKKN2 protein; multivalent protease inhibitor protein; WAP; follistatin/kazal; immunoglobulin; kunitz and netrin domain containing 2

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates

Detection Range

0.156 ng/ml-10 ng/ml.

Sensitivity

The minimum detectable dose of human WFIKKN2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human WFIKKN2. No significant cross-reactivity or interference between human WFIKKN2 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

63,941 Da

OMIM

610895

3D Structure

ModBase 3D Structure for Q8TEU8

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WFIKKN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WFIKKN2 purchase

MBS946744 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 (WFIKKN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WFIKKN2. The ELISA analytical biochemical technique of the MBS946744 kit is based on WFIKKN2 antibody-WFIKKN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WFIKKN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WFIKKN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WFIKKN2. It may not necessarily be applicable to this product.

NCBI GI #

28212222

NCBI GeneID

124857

NCBI Accession #

NP_783165.1

UniProt Primary Accession #

Q8TEU8

UniProt Secondary Accession #

Q6UXZ9

UniProt Related Accession #

Q8TEU8

GenBank Nucleotide #

NM_175575.5

NCBI Official Full Name

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

NCBI Official Synonym Full Names

WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2

NCBI Official Symbol

WFIKKN2

NCBI Official Synonym Symbols

GASP-1; WFDC20B; hGASP-1; WFIKKNRP

UniProt Protein Name

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

UniProt Synonym Protein Names

Growth and differentiation factor-associated serum protein 1; GASP-1; hGASP-1; WAP, follistatin, immunoglobulin, Kunitz and NTR domain-containing-related protein; WFIKKN-related protein

Protein Family

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein

UniProt Gene Name

WFIKKN2

UniProt Synonym Gene Names

GASP1; WFIKKNRP; UNQ9235/PRO31996; GASP-1; hGASP-1

UniProt Entry Name

WFKN2_HUMAN

NCBI Protein Information

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2; WFIKKN-related protein; WAP four-disulfide core domain 20B; multivalent protease inhibitor protein; WAP, FS, Ig, two KU and NTR module related protein; growth and differentiation factor-associated serum protein 1; WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-related protein

NCBI Summary for WFIKKN2

The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases. [provided by RefSeq, Jul 2008]

UniProt Comments for WFIKKN2

WFIKKN2: Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin. Belongs to the WFIKKN family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity

Biological Process: transforming growth factor beta receptor signaling pathway; palate development; negative regulation of protein binding; muscle fiber development; skeletal development; negative regulation of DNA binding

Research Articles on WFIKKN2

1. Data suggest that myostatin binding to its receptor (activin receptors type II) can be blocked by WFIKKN1 but not WFIKKN2; various forms of myostatin (promyostatin, myostatin pro-domain, and latent/processed myostatin) bind WFIKKN1 but not WFIKKN2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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