Secreted Phosphoprotein 2, 24kDa (SPP2) ELISA Kit for Human

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of SPP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS947110 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the secreted phosphoprotein 2, 24kDa (SPP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SPP2. The ELISA analytical biochemical technique of the MBS947110 kit is based on SPP2 antibody-SPP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SPP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).