Glutathione S-Transferase Omega 1 (GSTO1) ELISA Kit for Human

Name: Human GSTO1 ELISA Kit; Glutathione S-Transferase Omega 1; MyBioSource
Brand: MyBioSource
SKU: MBS947735
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS947735

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSTO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSTO1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

glutathione S-transferase omega 1 (GSTO1), ELISA Kit

Full Product Name

Human Glutathione S-transferase omega-1, GSTO1 ELISA Kit

Product Gene Name

GSTO1 elisa kit

Product Synonym Names

Human Glutathione S-transferase omega-1 (GSTO1) ELISA kit; RP11-99N20.1; DKFZp686H13163; GSTTLp28; P28; glutathione S-transferase omega 1-1; glutathione transferase omega 1; glutathione-S-transferase like; glutathione-S-transferase omega 1; glutathione S-transferase omega 1

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, plasma, tissue homogenates, Cell lysates

Detection Range

31.2 pg/ml -2000 pg/ml.

Sensitivity

The minimum detectable dose of human GSTO1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Specificity

This assay has high sensitivity and excellent specificity for detection of human GSTO1. No significant cross-reactivity or interference between human GSTO1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

27,566 Da

OMIM

605482

3D Structure

ModBase 3D Structure for P78417

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GSTO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSTO1 purchase

MBS947735 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutathione S-transferase omega 1 (GSTO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GSTO1. The ELISA analytical biochemical technique of the MBS947735 kit is based on GSTO1 antibody-GSTO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GSTO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GSTO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GSTO1. It may not necessarily be applicable to this product.

NCBI GI #

300360532

NCBI GeneID

9446

NCBI Accession #

NP_001177931.1

UniProt Primary Accession #

P78417

UniProt Secondary Accession #

Q5TA03; Q7Z3T2; D3DRA3; F5H7H0

UniProt Related Accession #

P78417

GenBank Nucleotide #

NM_001191002.1

NCBI Official Full Name

glutathione S-transferase omega-1 isoform 2

NCBI Official Synonym Full Names

glutathione S-transferase omega 1

NCBI Official Symbol

GSTO1

NCBI Official Synonym Symbols

P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21

UniProt Protein Name

Glutathione S-transferase omega-1

UniProt Synonym Protein Names

Glutathione S-transferase omega 1-1; GSTO 1-1; Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1); Monomethylarsonic acid reductase (EC:1.20.4.2); MMA(V) reductase; S-(Phenacyl)glutathione reductase; SPG-R

Protein Family

Glutathione S-transferase

UniProt Gene Name

GSTO1

UniProt Synonym Gene Names

GSTTLP28; GSTO-1; GSTO 1-1; MMA(V) reductase; SPG-R

UniProt Entry Name

GSTO1_HUMAN

NCBI Protein Information

glutathione S-transferase omega-1; GSTO-1; MMA(V) reductase; glutathione-S-transferase like; monomethylarsonic acid reductase; S-(Phenacyl)glutathione reductase; epididymis secretory protein Li 21; glutathione S-transferase omega 1-1; glutathione-dependent dehydroascorbate reductase

NCBI Summary for GSTO1

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

UniProt Comments for GSTO1

GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family.

Protein type: EC 1.20.4.2; EC 2.5.1.18; Transferase; Other Amino Acids Metabolism - glutathione; EC 1.8.5.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; glutathione transferase activity; glutathione dehydrogenase (ascorbate) activity; methylarsonate reductase activity; oxidoreductase activity

Biological Process: positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic metabolic process; L-ascorbic acid metabolic process; xenobiotic catabolic process

Research Articles on GSTO1

1. GSTT1 active genotype and GSTO1 Asp140Asp and GSTO2 Asp142Asp genotypes may have a prognostic/pharmacogenomic role in patients with muscle invasive bladder cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GSTO1 elisa kit

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Glutathione Conjugation Pathway antibodies	Glutathione Conjugation Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Vitamins And Cofactors Pathway antibodies	Metabolism Of Vitamins And Cofactors Pathway Diagram
Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies	Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram

Diseases associated with GSTO1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>24 publications with GSTO1 and Neoplasms
Brain Diseases Antibodies	>23 publications with GSTO1 and Brain Diseases
Carcinoma Antibodies	>11 publications with GSTO1 and Carcinoma
Parkinson Disease Antibodies	>10 publications with GSTO1 and Parkinson Disease
Cardiovascular Diseases Antibodies	>8 publications with GSTO1 and Cardiovascular Diseases
Skin Diseases Antibodies	>8 publications with GSTO1 and Skin Diseases
Breast Neoplasms Antibodies	>8 publications with GSTO1 and Breast Neoplasms
Vascular Diseases Antibodies	>7 publications with GSTO1 and Vascular Diseases
Lung Diseases Antibodies	>6 publications with GSTO1 and Lung Diseases
Atherosclerosis Antibodies	>6 publications with GSTO1 and Atherosclerosis

Organs/Tissues associated with GSTO1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>15 publications with GSTO1 and Liver
Blood Antibodies	>14 publications with GSTO1 and Blood
Brain Antibodies	>9 publications with GSTO1 and Brain
Vascular Antibodies	>5 publications with GSTO1 and Vascular
Lung Antibodies	>5 publications with GSTO1 and Lung
Heart Antibodies	>4 publications with GSTO1 and Heart
Prostate Antibodies	>4 publications with GSTO1 and Prostate
Muscle Antibodies	>3 publications with GSTO1 and Muscle
Kidney Antibodies	>3 publications with GSTO1 and Kidney
Thyroid Antibodies	>3 publications with GSTO1 and Thyroid