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Guanidinoacetate N-Methyltransferase (GAMT) Rat Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS949202

MBS949202 is a recombinant protein targeting Gamt. Also known as Recombinant Rat Guanidinoacetate N-methyltransferase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$335 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Guanidinoacetate N-methyltransferase (Gamt), Recombinant Protein
Full Product Name
Recombinant Rat Guanidinoacetate N-methyltransferase
Product Gene Name
Sequence
MSSSAASPLF APGEDCGPAW RAAPAAYDTS DTHLQILGKP VMERWETPYM HSLAAAAASR GGRVLEVGFG MAIAASRVQQ APIKEHWIIE CNDGVFQRLQ NWALKQPHKV VPLKGLWEEE APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKTHAFRLL KPGGILTYCN LTSWGELMKS KYTDITAMFE ETQVPALLEA GFQRENICTE VMALVPPADC RYYAFPQMIT PLVTKH
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Molecular Weight
30.4 kDa
Sequence Positions
1-236aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P10868
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for Gamt recombinant protein

Molecular cloning, sequence analysis, and expression in Escherichia coli of the cDNA for guanidinoacetate methyltransferase from rat liver.Ogawa H., Date T., Gomi T., Konishi K., Pitot H.C., Cantoni G.L., Fujioka M.Proc. Natl. Acad. Sci. U.S.A. 85:694-698(1988) Nucleotide sequence of the rat guanidinoacetate methyltransferase gene.Ogawa H., Fujioka M.Nucleic Acids Res. 16:8715-8716(1988) Recombinant rat guanidinoacetate methyltransferase structure and function of the NH2-terminal region as deduced by limited proteolysis.Fujioka M., Takata Y., Gomi T.Arch. Biochem. Biophys. 285:181-186(1991) Endogenous synthesis and transport of creatine in the rat brain an in situ hybridization study.Braissant O., Henry H., Loup M., Eilers B., Bachmann C.Brain Res. Mol. Brain Res. 86:193-201(2001) Creatine synthesis and transport during rat embryogenesis spatiotemporal expression of AGAT, GAMT and CT1.Braissant O., Henry H., Villard A.M., Speer O., Wallimann T., Bachmann C.BMC Dev. Biol. 5:9-9(2005) Crystal structure of guanidinoacetate methyltransferase from rat liver a model structure of protein arginine methyltransferase.Komoto J., Huang Y., Takata Y., Yamada T., Konishi K., Ogawa H., Gomi T., Fujioka M., Takusagawa F.J. Mol. Biol. 320:223-235(2002) Monoclinic guanidinoacetate methyltransferase and gadolinium ion-binding characteristics.Komoto J., Takata Y., Yamada T., Konishi K., Ogawa H., Gomi T., Fujioka M., Takusagawa F.Acta Crystallogr. D 59:1589-1596(2003) Catalytic mechanism of guanidinoacetate methyltransferase crystal structures of guanidinoacetate methyltransferase ternary complexes.Komoto J., Yamada T., Takata Y., Konishi K., Ogawa H., Gomi T., Fujioka M., Takusagawa F.Biochemistry 43:14385-14394(2004)

Other Notes

Small volumes of Gamt recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Gamt. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Guanidinoacetate N-methyltransferase
NCBI Official Synonym Full Names
guanidinoacetate N-methyltransferase
NCBI Official Symbol
Gamt
NCBI Official Synonym Symbols
GMT
UniProt Protein Name
Guanidinoacetate N-methyltransferase
UniProt Gene Name
Gamt
UniProt Entry Name
GAMT_RAT
NCBI Protein Information
guanidinoacetate N-methyltransferase

NCBI Summary for Gamt

catalyzes the last step of creatine biosynthesis [RGD, Feb 2006]

UniProt Comments for Gamt

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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