Human EDN1 Active Protein – Cat# MBS949552

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Endothelin-1 (EDN1) Human Active Protein

Name: EDN1 Active Protein; Endothelin-1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS949552
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS949552

MBS949552 is an active protein targeting EDN1. Also known as Recombinant Human Endothelin-1 (EDN1) (Active). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. Endothelins are endothelium-derived vasoconstrictor peptides. More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Endothelin-1 (EDN1), Active Protein

Full Product Name

Recombinant Human Endothelin-1 (EDN1) (Active)

Product Gene Name

EDN1 active protein

Product Synonym Names

Preproendothelin-1; PPET1

Sequence

CSCSSLMDKE CVYFCHLDII W

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Liquid or Lyophilized powder
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8

Purity/Purification

Greater than 90% as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

29.5 kDa

Sequence Positions

53-73aa; Full Length

OMIM

131240

3D Structure

ModBase 3D Structure for P05305

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Tag

N-terminal GST-tagged

Endotoxin

Not test.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 ug/ml can bind human EDN1 with a linear range of 6.25-25.00 ug/ml.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Product Categories/Family for EDN1 active protein

Signal Transduction

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of EDN1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EDN1. It may not necessarily be applicable to this product.

NCBI GI #

556203

NCBI GeneID

1906

NCBI Accession #

AAA52339.1

UniProt Primary Accession #

P05305

UniProt Related Accession #

P05305

NCBI Official Full Name

endothelin-1

NCBI Official Synonym Full Names

endothelin 1

NCBI Official Symbol

EDN1

NCBI Official Synonym Symbols

ET1; QME; PPET1; ARCND3; HDLCQ7

UniProt Protein Name

Endothelin-1

UniProt Synonym Protein Names

Preproendothelin-1

Protein Family

Endothelin

UniProt Gene Name

EDN1

UniProt Synonym Gene Names

PPET1; ET-1

UniProt Entry Name

EDN1_HUMAN

NCBI Protein Information

endothelin-1

NCBI Summary for EDN1

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

UniProt Comments for EDN1

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; response to amino acid stimulus; peptide hormone secretion; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; positive regulation of cell size; neural crest cell development; G-protein signaling, phospholipase D activating pathway; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; glucose transport; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; calcium-mediated signaling; positive regulation of hormone secretion; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; response to ozone; cartilage development; positive regulation of prostaglandin secretion; maternal process involved in parturition; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3

Research Articles on EDN1

1. Plasma levels of endothelin-1 is associated with increased coronary microvascular resistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EDN1 active protein

Reference Product	PubMed Publications
EDNRA active protein	>35 publications with EDN1 and EDNRA
EDNRB active protein	>25 publications with EDN1 and EDNRB
JUN active protein	>19 publications with EDN1 and JUN
ECE1 active protein	>14 publications with EDN1 and ECE1
FOS active protein	>2 publications with EDN1 and FOS
HIF1A active protein	>1 publications with EDN1 and HIF1A
GATA2 active protein	>1 publications with EDN1 and GATA2
AKT1 active protein	>1 publications with EDN1 and AKT1

Pathways associated with EDN1 active protein

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
EGFR-dependent Endothelin Signaling Events Pathway antibodies	EGFR-dependent Endothelin Signaling Events Pathway Diagram
Endothelin Pathway antibodies	Endothelin Pathway Diagram
Endothelins Pathway antibodies	Endothelins Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram

Diseases associated with EDN1 active protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>31 publications with EDN1 and Cardiovascular Diseases
Hypertension Antibodies	>26 publications with EDN1 and Hypertension
Craniofacial Abnormalities Antibodies	>16 publications with EDN1 and Craniofacial Abnormalities
Inflammation Antibodies	>15 publications with EDN1 and Inflammation
Asthma Antibodies	>13 publications with EDN1 and Asthma
Cardiovascular Abnormalities Antibodies	>6 publications with EDN1 and Cardiovascular Abnormalities
Kidney Diseases Antibodies	>6 publications with EDN1 and Kidney Diseases
Necrosis Antibodies	>5 publications with EDN1 and Necrosis
Heart Defects, Congenital Antibodies	>5 publications with EDN1 and Heart Defects, Congenital
Heart Failure Antibodies	>4 publications with EDN1 and Heart Failure

Organs/Tissues associated with EDN1 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>12 publications with EDN1 and Brain
Liver Antibodies	>3 publications with EDN1 and Liver